Irradiation induces up-regulation of E9 protein (CD105) in human vascular endothelial cells.
AffiliationChristie Hospital, Manchester, UK.
MetadataShow full item record
AbstractThe use of MAb E-9 raised against tissue-cultured endothelial cells (EC) has shown marked heterogeneity in vascular EC lining the blood vessels of normal and tumour tissues. MAb E-9 is human EC-specific and the protein recognized by it is a homodimer with a molecular mass of 97 kDa. The E-9 protein is resistant to treatment by 3 mM sodium periodate, but is sensitive to 10% trichloroacetic acid and 70% ethanol. E-9 protein has been assigned to a new cluster, CD105, and mapped to human chromosome 9q3. It has approximately 70% homology with type-III cell-surface receptor for transforming growth factor beta (TGF-beta). Recently CD105 has been reported to be the gene in patients with hereditary haemorrhagic telangiectasia. We have examined the effects of radiation on its expression in normal human umbilical-vein endothelial cells (HUVEC) and brain-tumour-derived endothelial cells (BTEC). Irradiation induced dose- and time-dependent up-regulation in the expression of the E-9 protein on the plasma membranes of EC, and also resulted in greater increase in the expression of the E-9 protein in semi-confluent (proliferating) as compared with confluent (non-proliferating) EC. It may well be that, following radiotherapy in cancer patients, E-9 protein is also up-regulated. The presence of increased amounts of E-9 protein in EC makes it an attractive target in the control of angiogenesis, especially after radiotherapy in cancer patients. The time scale involved in the up-regulation of E-9 protein following irradiation has led us to suggest that it may be a secondary event, the primary being the production and release of mitogenic factors (such as basic fibroblast growth factor) from irradiated EC.
CitationIrradiation induces up-regulation of E9 protein (CD105) in human vascular endothelial cells. 1995, 62 (6):791-6 Int. J. Cancer
JournalInternational Journal of Cancer
- Irradiation induces upregulation of CD31 in human endothelial cells.
- Authors: Quarmby S, Kumar P, Wang J, Macro JA, Hutchinson JJ, Hunter RD, Kumar S
- Issue date: 1999 Mar
- Antiangiogenic therapy of established tumors in human skin/severe combined immunodeficiency mouse chimeras by anti-endoglin (CD105) monoclonal antibodies, and synergy between anti-endoglin antibody and cyclophosphamide.
- Authors: Takahashi N, Haba A, Matsuno F, Seon BK
- Issue date: 2001 Nov 1
- Lack of specificity of endoglin expression for tumor blood vessels.
- Authors: Balza E, Castellani P, Zijlstra A, Neri D, Zardi L, Siri A
- Issue date: 2001 Nov
- Perfusion of 99Tcm-labeled CD105 Mab into kidneys from patients with renal carcinoma suggests that CD105 is a promising vascular target.
- Authors: Costello B, Li C, Duff S, Butterworth D, Khan A, Perkins M, Owens S, Al-Mowallad AF, O'Dwyer S, Kumar S
- Issue date: 2004 Apr 10
- Endoglin promotes endothelial cell proliferation and TGF-beta/ALK1 signal transduction.
- Authors: Lebrin F, Goumans MJ, Jonker L, Carvalho RL, Valdimarsdottir G, Thorikay M, Mummery C, Arthur HM, ten Dijke P
- Issue date: 2004 Oct 13