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    Localisation and cellular origin of hyaluronectin.

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    Authors
    Ponting, Julie M
    Kumar, Shant
    Affiliation
    Department of Clinical Research, Christie Hospital, Manchester, UK.
    Issue Date
    1995-10
    
    Metadata
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    Abstract
    Hyaluronectin is an extracellular matrix glycoprotein which specifically binds to hyaluronan. Isoforms of hyaluronectin are present in nervous and mesenchymal tissues but, while the nervous tissue isoform has been characterised in some detail, less is known about the mesenchymal isoform. Although its tissue localisation suggests a role in tumour development, neither its cellular origin nor its exact function are known. In this study we demonstrate hyaluronectin synthesis in fibroblasts and smooth muscle cells in vitro. The pattern of immunolocalisation of hyaluronectin in fibroblasts depended on the cell type, length of time spent by the cells in culture and cell density. Immunoreactivity in sparsely plated migratory cells was seen mainly in a patchy distribution at the attached cell surface and in the migration tracks left by the cells on the subtratum. In stationary cells a more uniform distribution associated with the attached cell surface was observed, while in confluent cultures hyaluronectin immunoreactivity was mainly seen as a network of fibrillar material above the cell. The pattern of staining was distinct from that of other hyaluronan-binding proteins. Immunoprecipitation, using antihyaluronectin antibodies, of the substratum-attached material deposited by human fetal fibroblasts revealed a family of proteins ranging from 22 to 90 kDa, the major protein being of approximately 60 kDa. These results lead us to propose that hyaluronectin plays an important role in cell migration, probably by regulation of hyaluronan distribution and binding.
    Citation
    Localisation and cellular origin of hyaluronectin. 1995, 187 ( Pt 2):331-46 J. Anat.
    Journal
    Journal of Anatomy
    URI
    http://hdl.handle.net/10541/99021
    PubMed ID
    7591996
    Type
    Article
    Language
    en
    ISSN
    0021-8782
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    All Christie Publications

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