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    Growth factor regulation of proliferation in primary cultures of small intestinal epithelium.

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    Authors
    Booth, Catherine
    Evans, Gareth S
    Potten, Christopher S
    Affiliation
    Department of Epithelial Biology, Paterson Institute for Cancer Research, Christie Hospital (NHS) Trust, Manchester, United Kingdom.
    Issue Date
    1995-03
    
    Metadata
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    Abstract
    Although the intestinal epithelium is one of the most rapidly renewing tissues, little is known about the major growth factors that control the rate of cell replacement and migration. Recently, a primary culture model has been described for the developing rat small intestinal epithelium, which permits epithelial growth while maintaining interactions with associated stromal cells, thereby possessing several contextual advantages over established cell lines (Evans et al., 1992). We have used this model to begin to determine the factors that may be involved in controlling intestinal epithelial cell proliferation. Under the conditions examined, no single growth factor promoted exclusive proliferation of epithelial cells; stromal cell proliferation was also apparent. The most potent stimulators of epithelial proliferation were insulin and insulin-like growth factor 1 (IGF-1). These factors also appeared to inhibit migration of the epithelial cells. 5-10 ng/ml EGF, 5-20 ng/ml TGF alpha, and 10-20 ng/ml PDGF also slightly increased epithelial cell numbers. Cell proliferation was inhibited by 0.1 ng/ml TGF beta-1. In Dulbecco's modified Eagle's medium (DMEM) containing 0.25 IU/ml insulin, glucose levels of 2-3 g/liter permitted epithelial growth with limited expansion of the stromal cell population. Higher levels of glucose further stimulated the nonepithelial cell types. Transferrin was also a potent stimulator of both cell types.
    Citation
    Growth factor regulation of proliferation in primary cultures of small intestinal epithelium. 1995, 31 (3):234-43 In Vitro Cell. Dev. Biol. Anim.
    Journal
    In Vitro Cellular & Developmental Biology. Animal
    URI
    http://hdl.handle.net/10541/98861
    DOI
    10.1007/BF02639439
    PubMed ID
    7757306
    Type
    Article
    Language
    en
    ISSN
    1071-2690
    ae974a485f413a2113503eed53cd6c53
    10.1007/BF02639439
    Scopus Count
    Collections
    All Paterson Institute for Cancer Research

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