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    Comparison of purity and enrichment of CD34+ cells from bone marrow, umbilical cord and peripheral blood (primed for apheresis) using five separation systems.

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    Authors
    De Wynter, Erika A
    Coutinho, Lucia H
    Pei, X
    Marsh, J C
    Hows, J
    Luft, T
    Testa, Nydia G
    Affiliation
    Cancer Research Campaign Department of Experimental Haematology, Paterson Institute for Cancer Research, Manchester, United Kingdom.
    Issue Date
    1995-09
    
    Metadata
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    Abstract
    Interest in the isolation and characterization of primitive hemopoietic cells in both the clinical and research fields has rapidly increased. In parallel, different purification systems have been developed to isolate these cells. We have compared five different methods for separation of CD34+ cells from human umbilical cord blood, normal bone marrow and apheresis harvests and analyzed purity, recovery, yield and enrichment of colony forming cells (CFC) for each individual system. Our results indicate that the most reliable methods of purification for all samples were fluorescence activated cell sorting (FACS) and magnetic activated cell sorting (MACS) which consistently yielded high purities (> 70%) and enrichment of CFC. In this respect the enrichment of CFC from the MACS was superior to all the other systems including FACS. Similar results (> 70%) for purity were obtained using avidin affinity columns and a biotinylated antibody but neither yield nor CFC enrichment approached the values achieved with MACS. On average CFC enrichment using these affinity columns was greater than that observed for FACS while the purity was comparable. Both CELLector flasks and immunomagnetic beads coated with CD34 antibodies were less effective in our hands in separating purified populations of progenitor cells. Both purity and CFC enrichment of CD34+ cells using these methods were at least 50% lower than obtained with either FACS, MACS or affinity columns.
    Citation
    Comparison of purity and enrichment of CD34+ cells from bone marrow, umbilical cord and peripheral blood (primed for apheresis) using five separation systems. 1995, 13 (5):524-32 Stem Cells
    Journal
    Stem Cells
    URI
    http://hdl.handle.net/10541/97963
    DOI
    10.1002/stem.5530130510
    PubMed ID
    8528102
    Type
    Article
    Language
    en
    ISSN
    1066-5099
    ae974a485f413a2113503eed53cd6c53
    10.1002/stem.5530130510
    Scopus Count
    Collections
    All Paterson Institute for Cancer Research

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