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dc.contributor.authorHoyes, Katherine P
dc.contributor.authorJohnson, C
dc.contributor.authorJohnston, R E
dc.contributor.authorLendon, R G
dc.contributor.authorHendry, Jolyon H
dc.contributor.authorSharma, H L
dc.contributor.authorMorris, Ian D
dc.date.accessioned2010-05-05T10:40:26Z
dc.date.available2010-05-05T10:40:26Z
dc.date.issued2010-05-05T10:40:26Z
dc.identifier.citationTesticular toxicity of the transferrin binding radionuclide 114mIn in adult and neonatal rats., 9 (3):297-305 Reprod. Toxicol.en
dc.identifier.issn0890-6238
dc.identifier.pmid7579915
dc.identifier.doi10.1016/0890-6238(95)00012-Y
dc.identifier.urihttp://hdl.handle.net/10541/97936
dc.description.abstractAdult (70 d) and neonatal (7 d) male rats were dosed (i.p.) with 37 MBq/kg (1 mCi/kg; approximately 1 microgram elemental indium/kg) 114mIn, a transferrin-binding radionuclide. In adults, approximately 0.25% of the injected activity localised within the testis by 48 h postinjection and remained constant for up to 63 d. In neonates, 0.06% of the activity was in the testis by 48 h, and this declined such that by 63 d only 0.03% remained. At 63 d, treated rats had reduced sperm head counts and abnormal testicular histology that was more marked in animals dosed as adults than as neonates. In vitro, uptake of 114mIn into seminiferous tubules isolated from 7-, 20-, or 70-d-old rats was compared with that of 125I. Both radionuclides were readily accumulated by the tubules. Whilst 114In uptake into 20- and 70-d tubules was inhibited by excess transferrin, uptake into 7-d tubules was unchanged. 125I uptake was not affected by excess transferrin. These data support the contention that some radionuclides may cross the blood-testis barrier by utilisation of the physiologic iron-transferrin pathway, which may lead to greater testicular damage in adult compared to neonatal animals.
dc.language.isoenen
dc.subject.meshAnimals
dc.subject.meshAnimals, Newborn
dc.subject.meshBody Weight
dc.subject.meshIndium Radioisotopes
dc.subject.meshMale
dc.subject.meshOrgan Size
dc.subject.meshProtein Binding
dc.subject.meshRats
dc.subject.meshRats, Sprague-Dawley
dc.subject.meshSeminiferous Tubules
dc.subject.meshSperm Count
dc.subject.meshSperm Head
dc.subject.meshTestis
dc.subject.meshTissue Distribution
dc.subject.meshTransferrin
dc.titleTesticular toxicity of the transferrin binding radionuclide 114mIn in adult and neonatal rats.en
dc.typeArticleen
dc.contributor.departmentCancer Research Campaign Department of Experimental Radiation Oncology, Paterson Institute for Cancer Research, Manchester, UK.en
dc.identifier.journalReproductive Toxicologyen
html.description.abstractAdult (70 d) and neonatal (7 d) male rats were dosed (i.p.) with 37 MBq/kg (1 mCi/kg; approximately 1 microgram elemental indium/kg) 114mIn, a transferrin-binding radionuclide. In adults, approximately 0.25% of the injected activity localised within the testis by 48 h postinjection and remained constant for up to 63 d. In neonates, 0.06% of the activity was in the testis by 48 h, and this declined such that by 63 d only 0.03% remained. At 63 d, treated rats had reduced sperm head counts and abnormal testicular histology that was more marked in animals dosed as adults than as neonates. In vitro, uptake of 114mIn into seminiferous tubules isolated from 7-, 20-, or 70-d-old rats was compared with that of 125I. Both radionuclides were readily accumulated by the tubules. Whilst 114In uptake into 20- and 70-d tubules was inhibited by excess transferrin, uptake into 7-d tubules was unchanged. 125I uptake was not affected by excess transferrin. These data support the contention that some radionuclides may cross the blood-testis barrier by utilisation of the physiologic iron-transferrin pathway, which may lead to greater testicular damage in adult compared to neonatal animals.


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