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dc.contributor.authorMunn, K E
dc.contributor.authorWalker, R A
dc.contributor.authorVarley, Jennifer
dc.date.accessioned2010-04-30T15:44:56Z
dc.date.available2010-04-30T15:44:56Z
dc.date.issued1995-04-20
dc.identifier.citationFrequent alterations of chromosome 1 in ductal carcinoma in situ of the breast. 1995, 10 (8):1653-7 Oncogeneen
dc.identifier.issn0950-9232
dc.identifier.pmid7731721
dc.identifier.urihttp://hdl.handle.net/10541/97777
dc.description.abstractDuctal carcinoma in situ (DCIS) of the breast is commonly described as a premalignant lesion. Using PCR to amplify DNA from areas of tumour cells which have been microdissected from fixed material, we have studied the involvement of chromosome 1 in 19 cases of DCIS. A series of microsatellite repeat polymorphisms has been used to define regions of allelic imbalance and this has confirmed the involvement in DCIS of six of the regions previously implicated in studies of invasive breast tumours. This suggests that these regions may harbour tumour suppressor genes, the inactivation of which is important for the early stages of breast tumour development. Analysis of separate ducts from within the same tumour has revealed that the same genetic alterations are not necessarily present throughout the lesion. In addition we have found that in three cases where frank invasive carcinoma is also present, similar alterations can be detected in the in situ and invasive component.
dc.language.isoenen
dc.subjectBreast Canceren
dc.subjectTumour Suppressor Genesen
dc.subject.meshBreast Neoplasms
dc.subject.meshCarcinoma in Situ
dc.subject.meshCarcinoma, Ductal, Breast
dc.subject.meshChromosome Aberrations
dc.subject.meshChromosomes, Human, Pair 1
dc.subject.meshFemale
dc.subject.meshGenes, Tumor Suppressor
dc.subject.meshHumans
dc.titleFrequent alterations of chromosome 1 in ductal carcinoma in situ of the breast.en
dc.typeArticleen
dc.contributor.departmentCRC Department of Cancer Genetics, Paterson Institute for Cancer Research, Christie Hospital, Manchester, UK.en
dc.identifier.journalOncogeneen
html.description.abstractDuctal carcinoma in situ (DCIS) of the breast is commonly described as a premalignant lesion. Using PCR to amplify DNA from areas of tumour cells which have been microdissected from fixed material, we have studied the involvement of chromosome 1 in 19 cases of DCIS. A series of microsatellite repeat polymorphisms has been used to define regions of allelic imbalance and this has confirmed the involvement in DCIS of six of the regions previously implicated in studies of invasive breast tumours. This suggests that these regions may harbour tumour suppressor genes, the inactivation of which is important for the early stages of breast tumour development. Analysis of separate ducts from within the same tumour has revealed that the same genetic alterations are not necessarily present throughout the lesion. In addition we have found that in three cases where frank invasive carcinoma is also present, similar alterations can be detected in the in situ and invasive component.


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