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    Discordant regulation of SCL/TAL-1 mRNA and protein during erythroid differentiation.

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    Authors
    Murrell, A M
    Bockamp, E O
    Göttgens, B
    Chan, Y S
    Cross, Michael A
    Heyworth, Clare M
    Green, A R
    Affiliation
    University of Cambridge, Department of Haematology, MRC Centre, UK.
    Issue Date
    1995-07-06
    
    Metadata
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    Abstract
    The SCL/TAL1 gene was originally identified by virtue of its rearrangement and transcriptional activation in patients with T cell acute lymphoblastic leukaemia. It encodes a helix-loop-helix transcription factor, is not normally expressed in T cells, but is expressed in erythroid, mast, megakaryocytic and progenitor cells. Over-expression of sense and antisense constructs have implicated SCL as a positive regulator of erythroid differentiation. In addition we have previously shown that SCL mRNA levels undergo biphasic modulation during induced erythroid differentiation of murine erythroleukaemia (MEL) cells with a transient early fall followed by a late rise. In this paper we have studied expression of the SCL protein during erythroid differentiation and also the molecular basis for the raised SCL mRNA levels that accompany erythroid differentiation. We have generated an anti-SCL antiserum and used it to demonstrate that an early transient fall in SCL protein does not occur during induced differentiation of MEL cells. Furthermore SCL protein levels underwent a late fall in three different models of erythroid differentiation and in two models of myeloid differentiation. The fall in SCL protein levels during induced erythroid differentiation contrasted with the concomitant marked rise in SCL mRNA levels. These observations have significant implications for the mechanism by which SCL may regulate erythropoiesis. In addition we have demonstrated that the stability of SCL mRNA was only marginally enhanced during erythroid differentiation of MEL cells, whereas the activity of a luciferase reporter construct driven by the SCL promoter was increased 11- to 17-fold. Up-regulation of transcription therefore accounted for most of the increase in SCL mRNA levels during erythroid differentiation.
    Citation
    Discordant regulation of SCL/TAL-1 mRNA and protein during erythroid differentiation. 1995, 11 (1):131-9 Oncogene
    Journal
    Oncogene
    URI
    http://hdl.handle.net/10541/97776
    PubMed ID
    7624120
    Type
    Article
    Language
    en
    ISSN
    0950-9232
    Collections
    All Paterson Institute for Cancer Research

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