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    The Epstein-Barr virus encoded cytokine viral interleukin-10 enhances transformation of human B lymphocytes.

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    Authors
    Stuart, Amanda D
    Stewart, James P
    Arrand, John R
    Mackett, Mike
    Affiliation
    Department of Molecular Biology, Paterson Institute for Cancer Research, Christie Hospital NHS Trust, Withington, Manchester.
    Issue Date
    1995-11-02
    
    Metadata
    Show full item record
    Abstract
    In vitro infection of human B lymphocytes with Epstein-Barr virus (EBV) results in their growth transformation and establishment of immortalised lymphoblastoid cell lines. The virus was recently found to encode a homologue of the pleitropic cytokine interleukin-10 (IL-10), which has wide ranging effects on the immune system. We have investigated the effect of this virally encoded growth factor on the ability of EBV to immortalize B lymphocytes from tonsils and from adult and neonatal blood. Recombinant viral interleukin-10 (vIL-10) was found to increase dramatically the growth transformation of B cells from all three populations infected with either the highly transforming type 1 strain B95-8 or the less efficient type 2 strain BL16. This striking enhancement of transforming ability in the presence of viral IL-10 may be in part due to increased viability of the B cells during infection and decreased levels of interferon-gamma, a cytokine known to inhibit EBV transformation. Thus viral IL-10 influences a number of cell types of the immune system to allow the enhanced outgrowth of EBV transformed cells.
    Citation
    The Epstein-Barr virus encoded cytokine viral interleukin-10 enhances transformation of human B lymphocytes. 1995, 11 (9):1711-9 Oncogene
    Journal
    Oncogene
    URI
    http://hdl.handle.net/10541/97769
    PubMed ID
    7478598
    Type
    Article
    Language
    en
    ISSN
    0950-9232
    Collections
    All Paterson Institute for Cancer Research

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