The Epstein-Barr virus encoded cytokine viral interleukin-10 enhances transformation of human B lymphocytes.
Affiliation
Department of Molecular Biology, Paterson Institute for Cancer Research, Christie Hospital NHS Trust, Withington, Manchester.Issue Date
1995-11-02
Metadata
Show full item recordAbstract
In vitro infection of human B lymphocytes with Epstein-Barr virus (EBV) results in their growth transformation and establishment of immortalised lymphoblastoid cell lines. The virus was recently found to encode a homologue of the pleitropic cytokine interleukin-10 (IL-10), which has wide ranging effects on the immune system. We have investigated the effect of this virally encoded growth factor on the ability of EBV to immortalize B lymphocytes from tonsils and from adult and neonatal blood. Recombinant viral interleukin-10 (vIL-10) was found to increase dramatically the growth transformation of B cells from all three populations infected with either the highly transforming type 1 strain B95-8 or the less efficient type 2 strain BL16. This striking enhancement of transforming ability in the presence of viral IL-10 may be in part due to increased viability of the B cells during infection and decreased levels of interferon-gamma, a cytokine known to inhibit EBV transformation. Thus viral IL-10 influences a number of cell types of the immune system to allow the enhanced outgrowth of EBV transformed cells.Citation
The Epstein-Barr virus encoded cytokine viral interleukin-10 enhances transformation of human B lymphocytes. 1995, 11 (9):1711-9 OncogeneJournal
OncogenePubMed ID
7478598Type
ArticleLanguage
enISSN
0950-9232Collections
Related articles
- Effect of cell-derived growth factors and cytokines on the clonal outgrowth of EBV-infected B cells and established lymphoblastoid cell lines.
- Authors: Ifversen P, Zhang XM, Ohlin M, Zeuthen J, Borrebaeck CA
- Issue date: 1993 Jul
- Improvement of EBV transformation and cloning efficiency of human B cells using culture supernatants from lymphoblastoid cell lines.
- Authors: Mevissen ML, Kwekkeboom J, Goormachtig E, Lindhout E, de Groot C
- Issue date: 1993 Apr
- Critical role of Epstein-Barr Virus (EBV)-encoded RNA in efficient EBV-induced B-lymphocyte growth transformation.
- Authors: Yajima M, Kanda T, Takada K
- Issue date: 2005 Apr
- In vitro transformation by Epstein-Barr virus induces a switch in growth factor and anti-IgM responsiveness in a human leukemic B cell clone.
- Authors: Janssen O, Gillis S, Kabelitz D
- Issue date: 1990 Jan
- Epstein-Barr virus (EBV)-encoded RNA 2 (EBER2) but not EBER1 plays a critical role in EBV-induced B-cell growth transformation.
- Authors: Wu Y, Maruo S, Yajima M, Kanda T, Takada K
- Issue date: 2007 Oct