• Login
    View Item 
    •   Home
    • The Manchester Institute Cancer Research UK
    • All Paterson Institute for Cancer Research
    • View Item
    •   Home
    • The Manchester Institute Cancer Research UK
    • All Paterson Institute for Cancer Research
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of ChristieCommunitiesTitleAuthorsIssue DateSubmit DateSubjectsThis CollectionTitleAuthorsIssue DateSubmit DateSubjectsProfilesView

    My Account

    LoginRegister

    Local Links

    The Christie WebsiteChristie Library and Knowledge Service

    Statistics

    Display statistics

    ogt alkyltransferase enhances dibromoalkane mutagenicity in excision repair-deficient Escherichia coli K-12.

    • CSV
    • RefMan
    • EndNote
    • BibTex
    • RefWorks
    Authors
    Abril, N
    Luque-Romero, F L
    Prieto-Alamo, M J
    Margison, Geoffrey P
    Pueyo, C
    Affiliation
    Departamento de Genética, Facultad de Ciencias, Universidad de Córdoba, Espana.
    Issue Date
    1995-02
    
    Metadata
    Show full item record
    Abstract
    We examined the role of the O6-alkylguanine-DNA alkyltransferase encoded by ogt gene in the sensitivity of Escherichia coli to the mutagenic effects of the dibromoalkanes, dibromoethane and dibromomethane, by comparing responses in ogt- bacteria to those in their isogenic ogt+ parental counterparts. The effects of the uvrABC excision-repair system, the adaptive response, mucAB and umuDC mutagenic processing, and glutathione bioactivation on the differential responses of ogt- and ogt+ bacteria were also studied. Mutation induction was monitored by measuring the frequency of forward mutations to L-arabinose resistance. Induced mutations occurred only in excision repair-defective strains and were totally (with dibromomethane) or substantially (with dibromoethane) dependent on the alkyltransferase (ATase) encoded by the ogt gene. An increased mutagenic response to both dibromoalkanes was also seen in ogt- bacteria that overexpressed the ogt protein from a multicopy plasmid, indicating that the differences in mutability between ogt+ and ogt- bacteria were not dependent on the ogt- null allele carried by the defective strain. The ATase encoded by the constitutive ogt gene was more effective in promoting dibromoalkane mutagenicity than the ada ATase induced by exposure to low doses of a methylating agent. The mutagenicity promoted by the ogt ATase was dependent on both glutathione bioactivation and SOS mutagenic processing. To our knowledge, this paper presents for the first time evidence that DNA ATases, in particular the ATase encoded by the ogt gene, can increase the mutagenic effects of a DNA-damaging agent. The mechanism of this effect has yet to be established.
    Citation
    ogt alkyltransferase enhances dibromoalkane mutagenicity in excision repair-deficient Escherichia coli K-12. 1995, 12 (2):110-7 Mol. Carcinog.
    Journal
    Molecular Carcinogenesis
    URI
    http://hdl.handle.net/10541/97752
    DOI
    10.1002/mc.2940120208
    PubMed ID
    7662116
    Type
    Article
    Language
    en
    ISSN
    0899-1987
    ae974a485f413a2113503eed53cd6c53
    10.1002/mc.2940120208
    Scopus Count
    Collections
    All Paterson Institute for Cancer Research

    entitlement

    Related articles

    • Bacterial and mammalian DNA alkyltransferases sensitize Escherichia coli to the lethal and mutagenic effects of dibromoalkanes.
    • Authors: Abril N, Luque-Romero FL, Prieto-Alamo MJ, Rafferty JA, Margison GP, Pueyo C
    • Issue date: 1997 Oct
    • Mutagenesis and DNA repair for alkylation damages in Escherichia coli K-12.
    • Authors: Abril N, Roldán-Arjona T, Prieto-Alamo MJ, van Zeeland AA, Pueyo C
    • Issue date: 1992
    • Mammalian cells expressing Escherichia coli O6-alkylguanine-DNA alkyltransferases are hypersensitive to dibromoalkanes.
    • Authors: Abril N, Margison GP
    • Issue date: 1999 Jun
    • Role of DNA repair by (A)BC excinuclease and Ogt alkyltransferase in the final distribution of LacI-d mutations induced by N-butyl-N-nitrosourea in Escherichia coli.
    • Authors: Ferrezuelo F, Prieto-Alamo MJ, Jurado J, Pueyo C
    • Issue date: 1998 Sep
    • Influence of DNA repair by (A)BC excinuclease and Ogt alkyltransferase on the distribution of mutations induced by n-propyl-N-nitrosourea in Escherichia coli.
    • Authors: Ferrezuelo F, Prieto-Alamo MJ, Jurado J, Pueyo C
    • Issue date: 1998
    DSpace software (copyright © 2002 - 2025)  DuraSpace
    Quick Guide | Contact Us
    Open Repository is a service operated by 
    Atmire NV
     

    Export search results

    The export option will allow you to export the current search results of the entered query to a file. Different formats are available for download. To export the items, click on the button corresponding with the preferred download format.

    By default, clicking on the export buttons will result in a download of the allowed maximum amount of items.

    To select a subset of the search results, click "Selective Export" button and make a selection of the items you want to export. The amount of items that can be exported at once is similarly restricted as the full export.

    After making a selection, click one of the export format buttons. The amount of items that will be exported is indicated in the bubble next to export format.