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    Role of interleukin-3 in the regulation of intracellular K+ homeostasis in cultured murine haemopoietic cells.

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    Authors
    Laskay, Gabor
    Várhelyi, T
    Dale, Robert E
    Dexter, T Michael
    Affiliation
    Department of Physics and Instrumentation, Paterson Institute for Cancer Research, Christie Hospital NHS Trust, Manchester, U.K.
    Issue Date
    1995-09-14
    
    Metadata
    Show full item record
    Abstract
    Comparative fluorimetric, flow cytofluorimetric and fluorescence ratiometric determinations of intracellular K+ concentrations in murine haemopoietic cells (FDCP-Mix clone A4) cultured in the presence and absence of the specific growth factor Interleukin-3 were carried out with the fluorescent potassium-binding benzofuran-isophthalate acetoxymethyl ester probe. Cell suspensions kept in the absence of Interleukin-3 for 5 hours exhibited lower fluorescence intensities and smaller fluorescence ratios than their growth-factor-replete counterparts, an effect found to be reversible by readdition of the growth factor. It is concluded that Interleukin-3 deprivation of these cells leads to loss of intracellular K+. It is tentatively suggested that this deprivation-induced K+ loss might be associated with an early event in apoptotic cell death.
    Citation
    Role of interleukin-3 in the regulation of intracellular K+ homeostasis in cultured murine haemopoietic cells. 1995, 214 (2):348-53 Biochem. Biophys. Res. Commun.
    Journal
    Biochemical and Biophysical Research Communications
    URI
    http://hdl.handle.net/10541/97495
    DOI
    10.1006/bbrc.1995.2294
    PubMed ID
    7677740
    Type
    Article
    Language
    en
    ISSN
    0006-291X
    ae974a485f413a2113503eed53cd6c53
    10.1006/bbrc.1995.2294
    Scopus Count
    Collections
    All Paterson Institute for Cancer Research

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