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    Macrophage inflammatory protein-1 alpha mediated growth inhibition in a haemopoietic stem cell line is associated with inositol 1,4,5 triphosphate generation.

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    Authors
    Heyworth, Clare M
    Pearson, Mark A
    Dexter, T Michael
    Wark, G
    Owen-Lynch, P J
    Whetton, Anthony D
    Affiliation
    Department of Experimental Haematology, Paterson Institute, Manchester, UK.
    Issue Date
    1995
    
    Metadata
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    Abstract
    Macrophage Inflammatory Protein-1 alpha (MIP-1 alpha) can inhibit the proliferation of multipotent haemopoietic cells. Using the FDCP-Mix A4 multipotent stem cell line, MIP-1 alpha was shown to inhibit 1L-3 stimulated cell cycling (assessed using the [3H]-thymidine "suicide" assay). Furthermore, MIP-1 alpha can inhibit 1L-3-stimulated [3H]-thymidine incorporation in FDCP-Mix cells, with half maximal inhibition observed at 3 ng/ml MIP-1 alpha. Prostaglandin E2, but not MIP-1 alpha was able to elevate cyclic AMP levels in FDCP-Mix A4 cells although both agents can cause growth inhibition. However, MIP-1 alpha addition resulted in a pertussis-toxin-insensitive increase in the level of the second messenger inositol 1,4,5 triphosphate (Ins 1,4,5P3). This response was both rapid (maximal at 5 seconds) and transient. A half maximal effect was observed at 5 ng/ml MIP-1 alpha and the dose dependency correlated with that for MIP-1 alpha mediated growth inhibition. A rapid increase in cytosolic Ca2+ levels was also observed in response to MIP-1 alpha. Inositol lipid hydrolysis and an increase in cytosolic Ca2+ (signals normally associated with proliferation) may therefore be implicated in growth inhibitory mechanisms in multipotent cells.
    Citation
    Macrophage inflammatory protein-1 alpha mediated growth inhibition in a haemopoietic stem cell line is associated with inositol 1,4,5 triphosphate generation. 1995, 12 (3):165-72 Growth Factors
    Journal
    Growth Factors
    URI
    http://hdl.handle.net/10541/97481
    DOI
    10.3109/08977199509036876
    PubMed ID
    8619922
    Type
    Article
    Language
    en
    ISSN
    0897-7194
    ae974a485f413a2113503eed53cd6c53
    10.3109/08977199509036876
    Scopus Count
    Collections
    All Paterson Institute for Cancer Research

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