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dc.contributor.authorKrizanac-Bengez, L
dc.contributor.authorBoranić, M
dc.contributor.authorTesta, Nydia G
dc.date.accessioned2010-04-27T14:36:26Z
dc.date.available2010-04-27T14:36:26Z
dc.date.issued1995
dc.identifier.citationNaloxone behaves as opioid agonist/antagonist in clonal cultures of mouse bone marrow cells. 1995, 49 (1):27-31 Biomed. Pharmacother.en
dc.identifier.issn0753-3322
dc.identifier.pmid7749076
dc.identifier.doi10.1016/0753-3322(96)82574-1
dc.identifier.urihttp://hdl.handle.net/10541/97464
dc.description.abstractThe opioid peptide methionine (Met)-enkephalin and the opioid-receptor blocking agent naloxone were added to unseparated or to progenitor-enriched cell suspensions of mouse bone marrow before assay in clonal cultures. Bone marrow samples harvested at 18:00 hours produced more granulocyte-macrophage (GM) colonies than the 06:00 hour samples, and were more sensitive to the proliferation inhibition by both agents. Additive inhibitory effects of naloxone with the enkephalin were occasionally seen. Thus, in this experimental system, naloxone could behave as an opioid agonist. However, there were examples of naloxone diminishing (blocking) the suppressive effect of the enkephalin, as a true opioid antagonist. Significant naloxone/enkephalin interactions occurred in opioid-sensitive (18:00 h) samples of unseparated bone marrow. The interactions were virtually absent in progenitor cell-enriched populations, indicating a significant role of accessory cells in opioidergic regulation of hematopoietic progenitors.
dc.language.isoenen
dc.subject.meshAnimals
dc.subject.meshBone Marrow Cells
dc.subject.meshCell Division
dc.subject.meshCells, Cultured
dc.subject.meshClone Cells
dc.subject.meshDrug Therapy, Combination
dc.subject.meshEnkephalin, Methionine
dc.subject.meshMale
dc.subject.meshMice
dc.subject.meshMice, Inbred C57BL
dc.subject.meshMice, Inbred DBA
dc.subject.meshNaloxone
dc.subject.meshStem Cells
dc.titleNaloxone behaves as opioid agonist/antagonist in clonal cultures of mouse bone marrow cells.en
dc.typeArticleen
dc.contributor.departmentRuder Bosković Institute, Zagreb, Croatia.en
dc.identifier.journalBiomedicine & Pharmacotherapyen
html.description.abstractThe opioid peptide methionine (Met)-enkephalin and the opioid-receptor blocking agent naloxone were added to unseparated or to progenitor-enriched cell suspensions of mouse bone marrow before assay in clonal cultures. Bone marrow samples harvested at 18:00 hours produced more granulocyte-macrophage (GM) colonies than the 06:00 hour samples, and were more sensitive to the proliferation inhibition by both agents. Additive inhibitory effects of naloxone with the enkephalin were occasionally seen. Thus, in this experimental system, naloxone could behave as an opioid agonist. However, there were examples of naloxone diminishing (blocking) the suppressive effect of the enkephalin, as a true opioid antagonist. Significant naloxone/enkephalin interactions occurred in opioid-sensitive (18:00 h) samples of unseparated bone marrow. The interactions were virtually absent in progenitor cell-enriched populations, indicating a significant role of accessory cells in opioidergic regulation of hematopoietic progenitors.


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