• Login
    View Item 
    •   Home
    • The Manchester Institute Cancer Research UK
    • All Paterson Institute for Cancer Research
    • View Item
    •   Home
    • The Manchester Institute Cancer Research UK
    • All Paterson Institute for Cancer Research
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of ChristieCommunitiesTitleAuthorsIssue DateSubmit DateSubjectsThis CollectionTitleAuthorsIssue DateSubmit DateSubjectsProfilesView

    My Account

    LoginRegister

    Local Links

    The Christie WebsiteChristie Library and Knowledge Service

    Statistics

    Display statistics

    Evidence that tenascin and thrombospondin-1 modulate sprouting of endothelial cells.

    • CSV
    • RefMan
    • EndNote
    • BibTex
    • RefWorks
    Authors
    Canfield, Ann E
    Schor, Ana M
    Affiliation
    CRC Department of Medical Oncology, Christie Hospital NHS Trust, Manchester, UK.
    Issue Date
    1995-02
    
    Metadata
    Show full item record
    Abstract
    Cultured endothelial cells undergo a reversible transition from a resting (cobblestone) phenotype to an angiogenic (sprouting) phenotype. This transition mimics the early events of angiogenesis. We have previously reported that the addition of exogenous xylosides inhibits endothelial cel sprouting and modifies the extracellular matrix (ECM) synthesised by the cells. We have now investigated whether endothelial sprouting is mediated by the nature of the extracellular matrix in contact with the cells. Accordingly, cell-free matrices deposited by bovine aortic endothelial cells (BAEC) were isolated. These matrices were produced under conditions in which the formation of the sprouting phenotype was permitted (controls) or inhibited (by the addition of exogenous xylosides). BAEC were then plated on these matrices and grown under conditions which promote sprouting. Sprouting proceeded normally on control matrices, whereas it was inhibited when the cells were grown on matrices deposited in the presence of xylosides. The composition of the permissive and inhibitory matrices was then analysed. Inhibitory matrices contained reduced levels of tenascin and increased levels of thrombospondin-1 by comparison to the permissive matrices. In contrast, no differences were detected in the relative levels of laminin. The roles of tenascin and thrombospondin-1 in endothelial sprouting were confirmed using specific antibodies. Immunolocalisation studies revealed the presence of both proteins in sprouting cells. Antibodies to tenascin inhibited the formation of sprouting cells on permissive matrices and on gelatin-coated dishes without affecting cell growth. Tenascin synthesis was increased when sprouting cells were present in the cultures. Antibodies to thrombospondin-1 stimulated sprouting on inhibitory matrices. These results suggest that the transition from a resting to a sprouting phenotype is promoted by tenascin and inhibited by thrombospondin-1.
    Citation
    Evidence that tenascin and thrombospondin-1 modulate sprouting of endothelial cells. 1995, 108 ( Pt 2):797-809 J. Cell. Sci.
    Journal
    Journal of Cell Science
    URI
    http://hdl.handle.net/10541/97403
    PubMed ID
    7539439
    Type
    Article
    Language
    en
    ISSN
    0021-9533
    Collections
    All Paterson Institute for Cancer Research

    entitlement

    Related articles

    • Thrombospondin gene expression by endothelial cells in culture is modulated by cell proliferation, cell shape and the substratum.
    • Authors: Canfield AE, Boot-Handford RP, Schor AM
    • Issue date: 1990 May 15
    • Heterogeneity in collagen biosynthesis by sprouting retinal endothelial cells.
    • Authors: Canfield AE, Schor AM
    • Issue date: 1994 Apr
    • Alpha- and beta-xylosides modulate the synthesis of fibronectin and thrombospondin-1 by endothelial cells.
    • Authors: Canfield AE, Sutton AB, Hiscock DR, Gallagher JT, Schor AM
    • Issue date: 1994 Aug 18
    • Extracellular proteins that modulate cell-matrix interactions. SPARC, tenascin, and thrombospondin.
    • Authors: Sage EH, Bornstein P
    • Issue date: 1991 Aug 15
    • The in situ localization of tenascin splice variants and thrombospondin 2 mRNA in the avian embryo.
    • Authors: Tucker RP
    • Issue date: 1993 Jan
    DSpace software (copyright © 2002 - 2025)  DuraSpace
    Quick Guide | Contact Us
    Open Repository is a service operated by 
    Atmire NV
     

    Export search results

    The export option will allow you to export the current search results of the entered query to a file. Different formats are available for download. To export the items, click on the button corresponding with the preferred download format.

    By default, clicking on the export buttons will result in a download of the allowed maximum amount of items.

    To select a subset of the search results, click "Selective Export" button and make a selection of the items you want to export. The amount of items that can be exported at once is similarly restricted as the full export.

    After making a selection, click one of the export format buttons. The amount of items that will be exported is indicated in the bubble next to export format.