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    Alpha- and beta-xylosides modulate the synthesis of fibronectin and thrombospondin-1 by endothelial cells.

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    Authors
    Canfield, Ann E
    Sutton, Andrew B
    Hiscock, Duncan R
    Gallagher, John T
    Schor, Ana M
    Affiliation
    CRC Department of Medical Oncology, Christie Hospital NHS Trust, Manchester, UK.
    Issue Date
    1994-08-18
    
    Metadata
    Show full item record
    Abstract
    We have previously shown that both p-nitrophenyl-alpha-D-xylopyranoside (alpha-xyloside) and p-nitrophenyl-beta-D-xylopyranoside (beta-xyloside) inhibit endothelial morphogenesis in vitro. In order to determine the mechanism for this inhibition, we have now investigated the effects of these compounds on the synthesis of proteoglycans and proteins by bovine aortic endothelial cells. Consistent with their well-recognised modes of action, beta-xyloside, but not alpha-xyloside, enhanced the secretion of free glycosaminoglycans into the medium. Furthermore, although both xylosides inhibited proteoglycan deposition into the cell layer/matrix, only beta-xyloside altered the nature of the proteoglycans synthesised by the cells. Both alpha- and beta-xylosides markedly inhibited total protein synthesis by endothelial cells in the absence of any effect on cell growth. This inhibition was time- and dose-dependent and was not due to the enzymatic release of p-nitrophenol by the cells. The synthesis of fibronectin and thrombospondin-1 were specifically and differentially modulated by both alpha- and beta-xylosides. That is, xylosides markedly reduced fibronectin levels relative to other proteins in both the medium and the cell layer/matrix. In contrast, the relative levels of thrombospondin-1 were increased in the xyloside-treated cultures both in terms of mRNA and protein. These studies demonstrate novel effects of xylosides on protein synthesis. Furthermore, they suggest that the inhibition of endothelial morphogenesis by xylosides may be due to the actions of these compounds on the synthesis of specific proteins.
    Citation
    Alpha- and beta-xylosides modulate the synthesis of fibronectin and thrombospondin-1 by endothelial cells. 1994, 1200 (3):249-58 Biochim. Biophys. Acta
    Journal
    Biochimica et Biophysica Acta
    URI
    http://hdl.handle.net/10541/97362
    PubMed ID
    8068710
    Type
    Article
    Language
    en
    ISSN
    0006-3002
    Collections
    All Christie Publications
    All Paterson Institute for Cancer Research

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