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    A reliable approach for sequencing clone-specific CDRIII regions in B-cell lymphoma.

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    Authors
    Straka, C
    Pettengell, Ruth
    Pielmeier, A
    Cross, Michael A
    Emmerich, B
    Crowther, Derek
    Testa, Nydia G
    Dexter, T Michael
    Affiliation
    Department of Experimental Haematology, C.R.C. Paterson Institute for Cancer Research, Manchester, UK.
    Issue Date
    1994
    
    Metadata
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    Abstract
    We report a reliable approach for sequencing lymphoma-specific CDRIII regions. CDRIII regions present in DNA prepared from routinely fixed and paraffin-embedded diagnostic lymph node material were amplified by the use of consensus VH and JH primers via PCR. PCR products were subcloned directly, without purification or modification of PCR fragments. Only small amounts of miniprep plasmid DNA of recombinant clones were required for cycle sequencing, resulting in autoradiograms of high quality. The easy and reproducible method which we describe has enabled us to determine the lymphoma-specific CDRIII region in 7/11 high-grade non-Hodgkin's lymphomas as well as in 3/3 cases of ALL and 1/1 case of a centroblastic/centrocytic lymphoma. The obtained sequence data can serve to generate lymphoma-specific oligonucleotides, which then can be used as PCR primers or hybridization probes for the detection of minimal residual disease in individual patients.
    Citation
    A reliable approach for sequencing clone-specific CDRIII regions in B-cell lymphoma. 1994, 5 Suppl 1:79-84 Ann. Oncol.
    Journal
    Annals of Oncology
    URI
    http://hdl.handle.net/10541/97346
    DOI
    10.1093/annonc/5.suppl_1.S79
    PubMed ID
    8172824
    Type
    Article
    Language
    en
    ISSN
    0923-7534
    ae974a485f413a2113503eed53cd6c53
    10.1093/annonc/5.suppl_1.S79
    Scopus Count
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    All Christie Publications
    All Paterson Institute for Cancer Research

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