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dc.contributor.authorPye, David A
dc.contributor.authorKumar, Shant
dc.contributor.authorWang, Ji Min
dc.contributor.authorHunter, Robin D
dc.date.accessioned2010-04-22T09:27:33Z
dc.date.available2010-04-22T09:27:33Z
dc.date.issued1994-02-17
dc.identifier.citationIrradiation of bovine aortic endothelial cells enhances the synthesis and secretion of sulphated glycosaminoglycans. 1994, 1220 (3):266-76 Biochim. Biophys. Actaen
dc.identifier.issn0006-3002
dc.identifier.pmid8305499
dc.identifier.urihttp://hdl.handle.net/10541/97133
dc.description.abstractThe effect of X-irradiation on the synthesis of heparan sulphate (HS) and chondroitin/dermatan sulphate (CS/DS) by bovine aortic endothelial cells (BAEC), was studied by measuring the incorporation of [35S]sulphate and [3H]glucosamine into newly synthesized glycosaminoglycan (GAG) chains. Medium extracts from irradiated cultures (5Gy) were found to contain approx. 130% more HS and 200% more CS/DS than unirradiated controls. Smaller increases were observed in cellular extracts, irradiated cultures (5Gy) containing approx. 60% more HS and 100% more CS/DS than unirradiated controls. Structural studies showed no significant changes occurred upon irradiation in either the amounts or distribution of N- and O-sulphate groups in the HS molecule. Values for N-sulphation of 41.1% control and 41.5% irradiated (5Gy) were obtained, the corresponding values for O-sulphation being 19.9% control and 20.2% irradiated. Isotope incorporation data indicated that sulphation of CS/DS may decrease after irradiation, however, analysis of chondroitin ABC lyase derived disaccharides showed no changes in the proportion of non-sulphated and O-sulphated disaccharides. The present study indicates that X-irradiation stimulates the synthesis and secretion of HS and CS/DS proteoglycans (PGs) by BAEC. This could be relevant to many features which are found to be indicative of radiation-induced damage.
dc.language.isoenen
dc.subject.meshAnimals
dc.subject.meshAorta
dc.subject.meshCattle
dc.subject.meshCells, Cultured
dc.subject.meshChromatography, Gel
dc.subject.meshChromatography, Ion Exchange
dc.subject.meshDose-Response Relationship, Radiation
dc.subject.meshElectrophoresis, Polyacrylamide Gel
dc.subject.meshEndothelium, Vascular
dc.subject.meshGlucosamine
dc.subject.meshGlycosaminoglycans
dc.subject.meshMolecular Weight
dc.subject.meshScintillation Counting
dc.subject.meshSulfates
dc.subject.meshSulfur Radioisotopes
dc.subject.meshTritium
dc.subject.meshX-Rays
dc.titleIrradiation of bovine aortic endothelial cells enhances the synthesis and secretion of sulphated glycosaminoglycans.en
dc.typeArticleen
dc.contributor.departmentDepartment of Clinical Research, Christie Hospital, Manchester, UK.en
dc.identifier.journalBiochimica et Biophysica Actaen
html.description.abstractThe effect of X-irradiation on the synthesis of heparan sulphate (HS) and chondroitin/dermatan sulphate (CS/DS) by bovine aortic endothelial cells (BAEC), was studied by measuring the incorporation of [35S]sulphate and [3H]glucosamine into newly synthesized glycosaminoglycan (GAG) chains. Medium extracts from irradiated cultures (5Gy) were found to contain approx. 130% more HS and 200% more CS/DS than unirradiated controls. Smaller increases were observed in cellular extracts, irradiated cultures (5Gy) containing approx. 60% more HS and 100% more CS/DS than unirradiated controls. Structural studies showed no significant changes occurred upon irradiation in either the amounts or distribution of N- and O-sulphate groups in the HS molecule. Values for N-sulphation of 41.1% control and 41.5% irradiated (5Gy) were obtained, the corresponding values for O-sulphation being 19.9% control and 20.2% irradiated. Isotope incorporation data indicated that sulphation of CS/DS may decrease after irradiation, however, analysis of chondroitin ABC lyase derived disaccharides showed no changes in the proportion of non-sulphated and O-sulphated disaccharides. The present study indicates that X-irradiation stimulates the synthesis and secretion of HS and CS/DS proteoglycans (PGs) by BAEC. This could be relevant to many features which are found to be indicative of radiation-induced damage.


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