Irradiation of bovine aortic endothelial cells enhances the synthesis and secretion of sulphated glycosaminoglycans.
dc.contributor.author | Pye, David A | |
dc.contributor.author | Kumar, Shant | |
dc.contributor.author | Wang, Ji Min | |
dc.contributor.author | Hunter, Robin D | |
dc.date.accessioned | 2010-04-22T09:27:33Z | |
dc.date.available | 2010-04-22T09:27:33Z | |
dc.date.issued | 1994-02-17 | |
dc.identifier.citation | Irradiation of bovine aortic endothelial cells enhances the synthesis and secretion of sulphated glycosaminoglycans. 1994, 1220 (3):266-76 Biochim. Biophys. Acta | en |
dc.identifier.issn | 0006-3002 | |
dc.identifier.pmid | 8305499 | |
dc.identifier.uri | http://hdl.handle.net/10541/97133 | |
dc.description.abstract | The effect of X-irradiation on the synthesis of heparan sulphate (HS) and chondroitin/dermatan sulphate (CS/DS) by bovine aortic endothelial cells (BAEC), was studied by measuring the incorporation of [35S]sulphate and [3H]glucosamine into newly synthesized glycosaminoglycan (GAG) chains. Medium extracts from irradiated cultures (5Gy) were found to contain approx. 130% more HS and 200% more CS/DS than unirradiated controls. Smaller increases were observed in cellular extracts, irradiated cultures (5Gy) containing approx. 60% more HS and 100% more CS/DS than unirradiated controls. Structural studies showed no significant changes occurred upon irradiation in either the amounts or distribution of N- and O-sulphate groups in the HS molecule. Values for N-sulphation of 41.1% control and 41.5% irradiated (5Gy) were obtained, the corresponding values for O-sulphation being 19.9% control and 20.2% irradiated. Isotope incorporation data indicated that sulphation of CS/DS may decrease after irradiation, however, analysis of chondroitin ABC lyase derived disaccharides showed no changes in the proportion of non-sulphated and O-sulphated disaccharides. The present study indicates that X-irradiation stimulates the synthesis and secretion of HS and CS/DS proteoglycans (PGs) by BAEC. This could be relevant to many features which are found to be indicative of radiation-induced damage. | |
dc.language.iso | en | en |
dc.subject.mesh | Animals | |
dc.subject.mesh | Aorta | |
dc.subject.mesh | Cattle | |
dc.subject.mesh | Cells, Cultured | |
dc.subject.mesh | Chromatography, Gel | |
dc.subject.mesh | Chromatography, Ion Exchange | |
dc.subject.mesh | Dose-Response Relationship, Radiation | |
dc.subject.mesh | Electrophoresis, Polyacrylamide Gel | |
dc.subject.mesh | Endothelium, Vascular | |
dc.subject.mesh | Glucosamine | |
dc.subject.mesh | Glycosaminoglycans | |
dc.subject.mesh | Molecular Weight | |
dc.subject.mesh | Scintillation Counting | |
dc.subject.mesh | Sulfates | |
dc.subject.mesh | Sulfur Radioisotopes | |
dc.subject.mesh | Tritium | |
dc.subject.mesh | X-Rays | |
dc.title | Irradiation of bovine aortic endothelial cells enhances the synthesis and secretion of sulphated glycosaminoglycans. | en |
dc.type | Article | en |
dc.contributor.department | Department of Clinical Research, Christie Hospital, Manchester, UK. | en |
dc.identifier.journal | Biochimica et Biophysica Acta | en |
html.description.abstract | The effect of X-irradiation on the synthesis of heparan sulphate (HS) and chondroitin/dermatan sulphate (CS/DS) by bovine aortic endothelial cells (BAEC), was studied by measuring the incorporation of [35S]sulphate and [3H]glucosamine into newly synthesized glycosaminoglycan (GAG) chains. Medium extracts from irradiated cultures (5Gy) were found to contain approx. 130% more HS and 200% more CS/DS than unirradiated controls. Smaller increases were observed in cellular extracts, irradiated cultures (5Gy) containing approx. 60% more HS and 100% more CS/DS than unirradiated controls. Structural studies showed no significant changes occurred upon irradiation in either the amounts or distribution of N- and O-sulphate groups in the HS molecule. Values for N-sulphation of 41.1% control and 41.5% irradiated (5Gy) were obtained, the corresponding values for O-sulphation being 19.9% control and 20.2% irradiated. Isotope incorporation data indicated that sulphation of CS/DS may decrease after irradiation, however, analysis of chondroitin ABC lyase derived disaccharides showed no changes in the proportion of non-sulphated and O-sulphated disaccharides. The present study indicates that X-irradiation stimulates the synthesis and secretion of HS and CS/DS proteoglycans (PGs) by BAEC. This could be relevant to many features which are found to be indicative of radiation-induced damage. |