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    Irradiation of bovine aortic endothelial cells enhances the synthesis and secretion of sulphated glycosaminoglycans.

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    Authors
    Pye, David A
    Kumar, Shant
    Wang, Ji Min
    Hunter, Robin D
    Affiliation
    Department of Clinical Research, Christie Hospital, Manchester, UK.
    Issue Date
    1994-02-17
    
    Metadata
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    Abstract
    The effect of X-irradiation on the synthesis of heparan sulphate (HS) and chondroitin/dermatan sulphate (CS/DS) by bovine aortic endothelial cells (BAEC), was studied by measuring the incorporation of [35S]sulphate and [3H]glucosamine into newly synthesized glycosaminoglycan (GAG) chains. Medium extracts from irradiated cultures (5Gy) were found to contain approx. 130% more HS and 200% more CS/DS than unirradiated controls. Smaller increases were observed in cellular extracts, irradiated cultures (5Gy) containing approx. 60% more HS and 100% more CS/DS than unirradiated controls. Structural studies showed no significant changes occurred upon irradiation in either the amounts or distribution of N- and O-sulphate groups in the HS molecule. Values for N-sulphation of 41.1% control and 41.5% irradiated (5Gy) were obtained, the corresponding values for O-sulphation being 19.9% control and 20.2% irradiated. Isotope incorporation data indicated that sulphation of CS/DS may decrease after irradiation, however, analysis of chondroitin ABC lyase derived disaccharides showed no changes in the proportion of non-sulphated and O-sulphated disaccharides. The present study indicates that X-irradiation stimulates the synthesis and secretion of HS and CS/DS proteoglycans (PGs) by BAEC. This could be relevant to many features which are found to be indicative of radiation-induced damage.
    Citation
    Irradiation of bovine aortic endothelial cells enhances the synthesis and secretion of sulphated glycosaminoglycans. 1994, 1220 (3):266-76 Biochim. Biophys. Acta
    Journal
    Biochimica et Biophysica Acta
    URI
    http://hdl.handle.net/10541/97133
    PubMed ID
    8305499
    Type
    Article
    Language
    en
    ISSN
    0006-3002
    Collections
    All Christie Publications

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