Differences in MHC and TAP-1 expression in cervical cancer lymph node metastases as compared with the primary tumours.
AuthorsCromme, F V
Walboomers, J M
Gallee, M P
Stern, Peter L
Helmerhorst, T J
Stukart, M J
Meijer, C J
AffiliationInstitute for Pathology, Free University Hospital, Amsterdam, The Netherlands.
MetadataShow full item record
AbstractIn previous studies we have shown down-regulation of class I major histocompatibility complex (MHC) expression in a significant proportion of primary cervical carcinomas, which was found to be strongly correlated with loss of expression of the transporter associated with antigen presentation (TAP). By contrast, class II MHC expression was frequently up-regulated on neoplastic keratinocytes in these malignancies. In order to investigate whether these changes are associated with biological behaviour of the tumours, 20 cervical carcinomas were analyzed for MHC (HLA-A, HLA-B/C, HLA-DR) and TAP-1 expression in the primary tumours and in lymph node metastases by immunohistochemistry. The results showed a significant increase in the prevalence of HLA-A and HLA-B/C down-regulation in metastasised neoplastic cells as compared with the primary tumour (P = 0.01). In all cases this was accompanied by loss of TAP-1 expression. Up-regulated HLA-DR expression was found exclusively in primary tumours and was absent in the corresponding metastases (P = 0.002). These data are consistent with the hypothesis that loss of TAP-1 and the consequent down-regulation of class I MHC expression provides a selective advantage for neoplastic cervical cells during metastasis. Furthermore, the lack of class II MHC expression in metastasised cells either reflects a different local lymphokine production or indicates that these cells may have escaped CD4+ cytotoxic T-lymphocyte (CTL)-mediated killing.
CitationDifferences in MHC and TAP-1 expression in cervical cancer lymph node metastases as compared with the primary tumours. 1994, 69 (6):1176-81 Br. J. Cancer
JournalBritish Journal of Cancer
- Loss of transporter protein, encoded by the TAP-1 gene, is highly correlated with loss of HLA expression in cervical carcinomas.
- Authors: Cromme FV, Airey J, Heemels MT, Ploegh HL, Keating PJ, Stern PL, Meijer CJ, Walboomers JM
- Issue date: 1994 Jan 1
- HLA class I expression and chromosomal deletions at 6p and 15q in head and neck squamous cell carcinomas.
- Authors: Feenstra M, Veltkamp M, van Kuik J, Wiertsema S, Slootweg P, van den Tweel J, de Weger R, Tilanus M
- Issue date: 1999 Sep
- Frequency of down-regulation of individual HLA-A and -B alleles in cervical carcinomas in relation to TAP-1 expression.
- Authors: Keating PJ, Cromme FV, Duggan-Keen M, Snijders PJ, Walboomers JM, Hunter RD, Dyer PA, Stern PL
- Issue date: 1995 Aug
- An immunohistochemical study of altered immunomodulatory molecule expression in head and neck squamous cell carcinoma.
- Authors: Vora AR, Rodgers S, Parker AJ, Start R, Rees RC, Murray AK
- Issue date: 1997
- Loss of transporter in antigen processing 1 transport protein and major histocompatibility complex class I molecules in metastatic versus primary breast cancer.
- Authors: Kaklamanis L, Leek R, Koukourakis M, Gatter KC, Harris AL
- Issue date: 1995 Nov 15