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dc.contributor.authorLawton, P Aen
dc.contributor.authorHodgkiss, R Jen
dc.contributor.authorEyden, Brian Pen
dc.contributor.authorJoiner, M Cen
dc.date.accessioned2010-04-21T14:26:32Z
dc.date.available2010-04-21T14:26:32Z
dc.date.issued1994-09
dc.identifier.citationGrowth of fibroblasts as a potential confounding factor in soft agar clonogenic assays for tumour cell radiosensitivity. 1994, 32 (3):218-25 Radiother Oncolen
dc.identifier.issn0167-8140
dc.identifier.pmid7529415
dc.identifier.doi10.1016/0167-8140(94)90021-3
dc.identifier.urihttp://hdl.handle.net/10541/97065
dc.description.abstractSoft agar clonogenic assays are considered to be a standard method for measuring tumour cell radiosensitivity and it has been widely reported that fibroblast contamination does not occur. We report here that human fibroblasts can proliferate to form colonies in a modified form of the Courtenay-Mills soft agar clonogenic assay. It was observed that early passage skin fibroblasts could form colonies in soft agar, although the plating efficiencies were reduced compared with growth on plastic. It was demonstrated that normal lung could proliferate in agar with similar plating efficiencies to fresh tumours and that fibroblastic cells were present in these cultures. Characterisation of primary lung tumour cultures also showed that fibroblastic cells were present in these cultures. Characterisation of primary lung tumour cultures also showed that fibroblastic cells were present which lacked epithelial features and which resembled closely the cells found in cultures of normal lung. This is an important finding for workers using soft agar assays to culture human tumour cells and is of interest in understanding the processes of normal growth control of human fibroblasts.
dc.language.isoenen
dc.subjectLung Canceren
dc.subjectCultured Tumour Cellsen
dc.subjectTumour Stem Cell Assayen
dc.subject.meshAgar
dc.subject.meshCell Division
dc.subject.meshCells, Cultured
dc.subject.meshCulture Media
dc.subject.meshEpithelial Cells
dc.subject.meshEpithelium
dc.subject.meshFibroblasts
dc.subject.meshHumans
dc.subject.meshKeratins
dc.subject.meshLung
dc.subject.meshLung Neoplasms
dc.subject.meshMicroscopy, Electron
dc.subject.meshRadiation Tolerance
dc.subject.meshSkin
dc.subject.meshTumor Cells, Cultured
dc.subject.meshTumor Stem Cell Assay
dc.titleGrowth of fibroblasts as a potential confounding factor in soft agar clonogenic assays for tumour cell radiosensitivity.en
dc.typeArticleen
dc.contributor.departmentCRC Gray Laboratory, Mount Vernon Hospital, Northwood, Middlesex, UK.en
dc.identifier.journalRadiotherapy and Oncologyen
html.description.abstractSoft agar clonogenic assays are considered to be a standard method for measuring tumour cell radiosensitivity and it has been widely reported that fibroblast contamination does not occur. We report here that human fibroblasts can proliferate to form colonies in a modified form of the Courtenay-Mills soft agar clonogenic assay. It was observed that early passage skin fibroblasts could form colonies in soft agar, although the plating efficiencies were reduced compared with growth on plastic. It was demonstrated that normal lung could proliferate in agar with similar plating efficiencies to fresh tumours and that fibroblastic cells were present in these cultures. Characterisation of primary lung tumour cultures also showed that fibroblastic cells were present in these cultures. Characterisation of primary lung tumour cultures also showed that fibroblastic cells were present which lacked epithelial features and which resembled closely the cells found in cultures of normal lung. This is an important finding for workers using soft agar assays to culture human tumour cells and is of interest in understanding the processes of normal growth control of human fibroblasts.


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