• Login
    View Item 
    •   Home
    • The Manchester Institute Cancer Research UK
    • All Paterson Institute for Cancer Research
    • View Item
    •   Home
    • The Manchester Institute Cancer Research UK
    • All Paterson Institute for Cancer Research
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of ChristieCommunitiesTitleAuthorsIssue DateSubmit DateSubjectsThis CollectionTitleAuthorsIssue DateSubmit DateSubjects

    My Account

    LoginRegister

    Local Links

    The Christie WebsiteChristie Library and Knowledge Service

    Statistics

    Display statistics

    An E. coli ada transgenic clone of Nicotiana tabacum var. Xanthi has increased sensitivity to the mutagenic action of alkylating agents, maleic hydrazide and gamma-rays.

    • CSV
    • RefMan
    • EndNote
    • BibTex
    • RefWorks
    Authors
    Velemínský, J
    Angelis, K
    Babůrek, I
    Gichner, T
    Satava, J
    Bríza, J
    Margison, Geoffrey P
    Affiliation
    Institute of Experimental Botany, Academy of Sciences, Prague, Czech Republic.
    Issue Date
    1994-05-01
    
    Metadata
    Show full item record
    Abstract
    Two transgenic clones X3 and X15 of Nicotiana tabacum var. Xanthi, heterozygous in two genes (a1 and a2) for chloroplast differentiation and transformed with the E. coli DNA repair gene ada cloned downstream from the 1' direction of the dual mas promoter, differed in the expression of the ada gene, in the number of copies of integrated T-DNA and in the response to the mutagenic action of alkylating and non-alkylating agents. The X3 genome contained four copies and the X15 genome one copy of T-DNA, nevertheless the expression of the ada gene, measured by the activity of O6-alkylguanine DNA alkyltransferase (ATase), was about six times higher in X15 than in X3. ATase activity in both clones was highest in extracts from callus whereas very low (X15) or no (X3) activity was detected in leaf extracts. This may explain the lack of difference between X15 and non-transformed tobacco (NTX) in the frequency of N-methyl-N-nitrosourea (MNU)-induced somatic mutations in leaves. In contrast, the frequency of somatic mutations in X3 was about 2-5 times higher than in NTX and X15 after the same doses of MNU, methyl methanesulfonate, maleic hydrazide and gamma-rays. Alteration of plant gene(s) essential in mutation pathway(s) by insertion of T-DNA or by somaclonal variation may explain the higher sensitivity of the X3 clone.
    Citation
    An E. coli ada transgenic clone of Nicotiana tabacum var. Xanthi has increased sensitivity to the mutagenic action of alkylating agents, maleic hydrazide and gamma-rays. 1994, 307 (1):193-200 Mutat. Res.
    Journal
    Mutation Research
    URI
    http://hdl.handle.net/10541/96973
    PubMed ID
    7513797
    Type
    Article
    Language
    en
    ISSN
    0027-5107
    Collections
    All Paterson Institute for Cancer Research

    entitlement

    Related articles

    • Increased resistance to the toxic effects of alkylating agents in tobacco expressing the E. coli DNA repair gene ada.
    • Authors: Angelis K, Bríza J, Satava J, Skákal I, Velemínský J, Vlasák J, Kleibl K, Margison GP
    • Issue date: 1992 May
    • Expression in mammalian cells of the Escherichia coli O6 alkylguanine-DNA-alkyltransferase gene ogt reduces the toxicity of alkylnitrosoureas.
    • Authors: Harris LC, Margison GP
    • Issue date: 1993 Jun
    • Cloning and characterization of the Salmonella typhimurium ada gene, which encodes O6-methylguanine-DNA methyltransferase.
    • Authors: Hakura A, Morimoto K, Sofuni T, Nohmi T
    • Issue date: 1991 Jun
    • Influence of DNA repair by ada and ogt alkyltransferases on the mutational specificity of alkylating agents.
    • Authors: Roldán-Arjona T, Luque-Romero FL, Ariza RR, Jurado J, Pueyo C
    • Issue date: 1994 Apr
    • Transfection and expression of human O6-methylguanine-DNA methyltransferase (MGMT) cDNA in Chinese hamster cells: the role of MGMT in protection against the genotoxic effects of alkylating agents.
    • Authors: Kaina B, Fritz G, Mitra S, Coquerelle T
    • Issue date: 1991 Oct
    DSpace software (copyright © 2002 - 2021)  DuraSpace
    Quick Guide | Contact Us
    Open Repository is a service operated by 
    Atmire NV
     

    Export search results

    The export option will allow you to export the current search results of the entered query to a file. Different formats are available for download. To export the items, click on the button corresponding with the preferred download format.

    By default, clicking on the export buttons will result in a download of the allowed maximum amount of items.

    To select a subset of the search results, click "Selective Export" button and make a selection of the items you want to export. The amount of items that can be exported at once is similarly restricted as the full export.

    After making a selection, click one of the export format buttons. The amount of items that will be exported is indicated in the bubble next to export format.