Biological consequences of p160v-abl protein tyrosine kinase activity in a primitive, multipotent haemopoietic cell line.
Fairbairn, Leslie J
Cowling, Graham J
Dexter, T Michael
Whetton, Anthony D
Owen-Lynch, P Jane
AffiliationDepartment of Experimental Haematology, Paterson Institute for Cancer Research, Christie Hospital NHS Trust, Manchester, UK.
MetadataShow full item record
AbstractA temperature sensitive abl protein tyrosine kinase gene was transferred into a multipotent haemopoietic stem cell line, and the primary biological effects of expression of the gene were examined at the permissive and non-permissive temperatures. Unlike previous studies in factor-dependent cell lines, we found that expression of the functional abl protein tyrosine kinase did not lead to growth autonomy. Furthermore, the cells were still able to undergo terminal myeloid differentiation. However, expression of the functional gene did lead to a delay in maturation with a concomitant increase in cell production, had a modest effect in terms of delayed apoptosis particularly when the cells were maintained at a high cell density, and slightly increased the response to sub-optimal concentrations of IL-3. In many respects, therefore, the effects of abl protein tyrosine kinase in these cells mimics the effect of bcr/abl in primary haemopoietic cells where growth factor independence and an aberrant differentiation profile are relatively late events in clonal evolution and are not intermediate consequences of activation of the abl gene.
CitationBiological consequences of p160v-abl protein tyrosine kinase activity in a primitive, multipotent haemopoietic cell line. 1994, 8 (4):620-30 Leukemia
- Abl protein kinase abrogates the response of multipotent haemopoietic cells to the growth inhibitor macrophage inflammatory protein-1 alpha.
- Authors: Wark G, Heyworth CM, Spooncer E, Czaplewski L, Francis JM, Dexter TM, Whetton AD
- Issue date: 1998 Mar 12
- v-Abl-mediated apoptotic suppression is associated with SHC phosphorylation without concomitant mitogen-activated protein kinase activation.
- Authors: Owen-Lynch PJ, Wong AK, Whetton AD
- Issue date: 1995 Mar 17
- Role of acid/base homeostasis in the suppression of apoptosis in haemopoietic cells by v-Abl protein tyrosine kinase.
- Authors: Chen Q, Benson RS, Whetton AD, Brant SR, Donowitz M, Montrose MH, Dive C, Watson AJ
- Issue date: 1997 Feb
- p210 Bcr-Abl expression in a primitive multipotent haematopoietic cell line models the development of chronic myeloid leukaemia.
- Authors: Pierce A, Owen-Lynch PJ, Spooncer E, Dexter TM, Whetton AD
- Issue date: 1998 Aug 6
- Bcr-Abl-mediated molecular mechanism for apoptotic suppression in multipotent haemopoietic cells: a role for PKCbetaII.
- Authors: Xenaki D, Pierce A, Underhill-Day N, Whetton AD, Owen-Lynch PJ
- Issue date: 2004 Feb