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    Clonogen content of intestinal crypts: its deduction using a microcolony assay on whole mount preparations and its dependence on radiation dose.

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    Authors
    Roberts, Stephen A
    Potten, Christopher S
    Affiliation
    Cancer Research Campaign, Paterson Institute for Cancer Research, Christie Hospital, Withington, Manchester, UK.
    Issue Date
    1994-04
    
    Metadata
    Show full item record
    Abstract
    A new method for detecting surviving intestinal microcolonies in whole mount preparations is presented. This approach is not subject to the sampling problems encountered with microcolony assay using data from tissue sections, associated with the size of the microcolonies and the random nature of the sections in relation to these colonies. This new method has been used to determine the crypt survival after one or two fractions of gamma-irradiation, and hence to estimate the crypt clonogen number. The survival levels are similar using the two techniques and similar estimates for the number of clonogens per crypt are obtained. The results using this novel approach thus confirm the validity of the traditional approaches using sections. The estimates of initial clonogen number per crypt show a dependence on the size of dose used to estimate the number, and the estimates range from about eight clonogens per crypt for doses between 7 and 8 Gy, to about 40 clonogens per crypt for doses between 9 and 10 Gy.
    Citation
    Clonogen content of intestinal crypts: its deduction using a microcolony assay on whole mount preparations and its dependence on radiation dose. 1994, 65 (4):477-81 Int. J. Radiat. Biol.
    Journal
    International Journal of Radiation Biology
    URI
    http://hdl.handle.net/10541/96216
    DOI
    10.1080/09553009414550551
    PubMed ID
    7908935
    Type
    Article
    Language
    en
    ISSN
    0955-3002
    ae974a485f413a2113503eed53cd6c53
    10.1080/09553009414550551
    Scopus Count
    Collections
    All Paterson Institute for Cancer Research

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    Related articles

    • Deduction of the clonogen content of intestinal crypts: a direct comparison of two-dose and multiple-dose methodologies.
    • Authors: Roberts SA, Hendry JH, Potten CS
    • Issue date: 1995 Mar
    • Intestinal crypt clonogens: a new interpretation of radiation survival curve shape and clonogenic cell number.
    • Authors: Roberts SA, Hendry JH, Potten CS
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    • The effect of proliferative status and clonogen content on the response of mouse jejunal crypts to split-dose irradiation.
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    • Issue date: 1997 Feb
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    • Changes in clonogen number and radiation sensitivity in mouse jejunal crypts after treatment with dimethylsulfoxide and retinoic acid.
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