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    Colonic mucosal replacement by syngeneic small intestinal stem cell transplantation.

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    Authors
    Tait, I S
    Evans, Gareth S
    Flint, Neil
    Campbell, F C
    Affiliation
    University Department of Surgery, Ninewells Hospital and Medical School, Dundee, Scotland.
    Issue Date
    1994-01
    
    Metadata
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    Abstract
    A novel method of colonic mucosal replacement by transplantation of disaggregated small intestinal epithelium is described. Thirty-one inbred rats had the ascending colon isolated, and surgical mucosectomy was performed on the "free" loop. Epithelial cell aggregates were isolated from postnatal small intestine using collagenase and dispase digestion, then 20 microL of the cell suspension was "seeded" over the denuded colonic muscle of 25 recipient rats. Six control rats had surgical mucosectomy only. All loops were retrieved after 14 days for histologic examination. Stem cell lineage studies were used with selective staining protocols to identify enterocytes, goblet cells, entero-endocrine cells, and Paneth cells. A neomucosa with typical small bowel morphology including crypts and villi and all four stem cell lineages was regenerated by transplanted cells on the colonic muscle in 19 of 25 (76%) recipients. Control loops showed no epithelial regrowth confirming total mucosectomy. With appropriate stromal support, transplanted small intestinal stem cells have the capacity to re-epithelialize denuded colonic muscle with small bowel neomucosa.
    Citation
    Colonic mucosal replacement by syngeneic small intestinal stem cell transplantation. 1994, 167 (1):67-72 Am. J. Surg.
    Journal
    American Journal of Surgery
    URI
    http://hdl.handle.net/10541/96205
    DOI
    10.1016/0002-9610(94)90055-8
    PubMed ID
    8311142
    Type
    Article
    Language
    en
    ISSN
    0002-9610
    ae974a485f413a2113503eed53cd6c53
    10.1016/0002-9610(94)90055-8
    Scopus Count
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    All Paterson Institute for Cancer Research

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