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dc.contributor.authorPendlebury, Alizon
dc.contributor.authorFrayling, Ian M
dc.contributor.authorSantibanez-Koref, Mauro F
dc.contributor.authorMargison, Geoffrey P
dc.contributor.authorRafferty, Joseph A
dc.date.accessioned2010-04-09T13:49:39Z
dc.date.available2010-04-09T13:49:39Z
dc.date.issued1994-12
dc.identifier.citationEvidence for the simultaneous expression of alternatively spliced alkylpurine N-glycosylase transcripts in human tissues and cells. 1994, 15 (12):2957-60 Carcinogenesisen
dc.identifier.issn0143-3334
dc.identifier.pmid8001263
dc.identifier.doi10.1093/carcin/15.12.2957
dc.identifier.urihttp://hdl.handle.net/10541/96196
dc.description.abstractWe have isolated a novel human alkylpurine N-glycosylase (APNG) cDNA from a placental library by screening with an oligonucleotide based on the published sequence of the human liver cDNA encoding this protein. The nucleotide sequences of the two cDNAs were essentially identical, but the 5' untranslated region of the new sequence was truncated and the 5'-terminal 92 nucleotides of the novel cDNA were different, indicating the possibility of alternative transcripts. This region included a portion of the open reading frame, so that the predicted protein was truncated and the seven N-terminal amino acids differed from the published sequence for APNG. PCR amplification of reverse transcribed mRNA, using 5' primers unique to the two cDNAs and a common 3' primer showed that the alternative transcripts can be co-expressed in the same cells and tissues.
dc.language.isoenen
dc.subject.meshAmino Acid Sequence
dc.subject.meshBase Sequence
dc.subject.meshCell Line
dc.subject.meshDNA, Complementary
dc.subject.meshGene Expression Regulation, Enzymologic
dc.subject.meshHumans
dc.subject.meshIsoenzymes
dc.subject.meshMolecular Sequence Data
dc.subject.meshOrgan Specificity
dc.subject.meshRNA Splicing
dc.subject.meshRNA, Heterogeneous Nuclear
dc.subject.meshRNA, Messenger
dc.subject.meshSequence Alignment
dc.subject.meshSequence Homology, Amino Acid
dc.subject.meshSubstrate Specificity
dc.titleEvidence for the simultaneous expression of alternatively spliced alkylpurine N-glycosylase transcripts in human tissues and cells.en
dc.typeArticleen
dc.contributor.departmentCRC Department of Carcinogenesis, Paterson Institute for Cancer Research, Christie Hospital-NHS Trust, Manchester, UK.en
dc.identifier.journalCarcinogenesisen
html.description.abstractWe have isolated a novel human alkylpurine N-glycosylase (APNG) cDNA from a placental library by screening with an oligonucleotide based on the published sequence of the human liver cDNA encoding this protein. The nucleotide sequences of the two cDNAs were essentially identical, but the 5' untranslated region of the new sequence was truncated and the 5'-terminal 92 nucleotides of the novel cDNA were different, indicating the possibility of alternative transcripts. This region included a portion of the open reading frame, so that the predicted protein was truncated and the seven N-terminal amino acids differed from the published sequence for APNG. PCR amplification of reverse transcribed mRNA, using 5' primers unique to the two cDNAs and a common 3' primer showed that the alternative transcripts can be co-expressed in the same cells and tissues.


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