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dc.contributor.authorTarpey, I
dc.contributor.authorStacey, Simon N
dc.contributor.authorHickling, J
dc.contributor.authorBirley, H D
dc.contributor.authorRenton, A
dc.contributor.authorMcIndoe, A
dc.contributor.authorDavies, D H
dc.date.accessioned2010-04-09T11:04:14Z
dc.date.available2010-04-09T11:04:14Z
dc.date.issued1994-02
dc.identifier.citationHuman cytotoxic T lymphocytes stimulated by endogenously processed human papillomavirus type 11 E7 recognize a peptide containing a HLA-A2 (A*0201) motif. 1994, 81 (2):222-7 Immunologyen
dc.identifier.issn0019-2805
dc.identifier.pmid8157270
dc.identifier.urihttp://hdl.handle.net/10541/96137
dc.description.abstractCytotoxic T lymphocytes (CTL) may play an important role in the control of human papillomavirus (HPV)-induced anogenital neoplasias, but have been difficult to study owing to the difficulty in obtaining sufficient quantities of infectious virus. To address this we have stimulated human HPV-specific CTL in vitro using low-density cells (LDC) from peripheral blood mononuclear cells (PBMC). Low-density cells were used to present synthetic peptides, or endogenously processed peptides expressed from recombinant vaccinia viruses, to high-density PBMC (predominantly lymphocytes) for 6 days. Cytotoxic T lymphocytes stimulated with endogenously processed HPV 11 E7 recognized the synthetic HLA-A2 (A*0201) motif-containing nonamer, 4-12. In reciprocal experiments, CTL stimulated with this peptide in vitro recognized targets expressing endogenously processed E7. The responses in each case were A2 restricted and peptide specific. Two additional A2 motif-containing nonamers from HPV 6b E7 (21-30 and 47-55) also elicited peptide-specific, A2-restricted CTL. The data illustrate the potential that in vitro stimulation with LDC has in understanding CTL responses to experimentally problematic viral systems such as HPV, and may offer a route to specific immunotherapy of HPV-associated lesions.
dc.language.isoenen
dc.subject.meshAmino Acid Sequence
dc.subject.meshAntigens, Viral
dc.subject.meshBlotting, Northern
dc.subject.meshCells, Cultured
dc.subject.meshHLA-A2 Antigen
dc.subject.meshHumans
dc.subject.meshLeukocytes, Mononuclear
dc.subject.meshMolecular Sequence Data
dc.subject.meshPapillomaviridae
dc.subject.meshPeptides
dc.subject.meshRecombination, Genetic
dc.subject.meshT-Lymphocytes, Cytotoxic
dc.subject.meshVaccinia virus
dc.titleHuman cytotoxic T lymphocytes stimulated by endogenously processed human papillomavirus type 11 E7 recognize a peptide containing a HLA-A2 (A*0201) motif.en
dc.typeArticleen
dc.contributor.departmentDivision of Life Sciences, King's College, London, U.K.en
dc.identifier.journalImmunologyen
html.description.abstractCytotoxic T lymphocytes (CTL) may play an important role in the control of human papillomavirus (HPV)-induced anogenital neoplasias, but have been difficult to study owing to the difficulty in obtaining sufficient quantities of infectious virus. To address this we have stimulated human HPV-specific CTL in vitro using low-density cells (LDC) from peripheral blood mononuclear cells (PBMC). Low-density cells were used to present synthetic peptides, or endogenously processed peptides expressed from recombinant vaccinia viruses, to high-density PBMC (predominantly lymphocytes) for 6 days. Cytotoxic T lymphocytes stimulated with endogenously processed HPV 11 E7 recognized the synthetic HLA-A2 (A*0201) motif-containing nonamer, 4-12. In reciprocal experiments, CTL stimulated with this peptide in vitro recognized targets expressing endogenously processed E7. The responses in each case were A2 restricted and peptide specific. Two additional A2 motif-containing nonamers from HPV 6b E7 (21-30 and 47-55) also elicited peptide-specific, A2-restricted CTL. The data illustrate the potential that in vitro stimulation with LDC has in understanding CTL responses to experimentally problematic viral systems such as HPV, and may offer a route to specific immunotherapy of HPV-associated lesions.


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