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dc.contributor.authorMyers, Kevin A
dc.contributor.authorRahi-Saund, Veena
dc.contributor.authorDavison, M D
dc.contributor.authorYoung, J A
dc.contributor.authorCheater, Amanada J
dc.contributor.authorStern, Peter L
dc.date.accessioned2010-04-09T11:02:29Z
dc.date.available2010-04-09T11:02:29Z
dc.date.issued1994-03-25
dc.identifier.citationIsolation of a cDNA encoding 5T4 oncofetal trophoblast glycoprotein. An antigen associated with metastasis contains leucine-rich repeats. 1994, 269 (12):9319-24 J. Biol. Chem.en
dc.identifier.issn0021-9258
dc.identifier.pmid8132670
dc.identifier.urihttp://hdl.handle.net/10541/96136
dc.description.abstractThe monoclonal antibody 5T4 defines a human oncotrophoblast antigen expressed by a variety of carcinomas but with a restricted pattern of expression in normal adult tissues. The 5T4 antigen has been isolated from term placenta as a 72-kDa glycoprotein consisting of a 42-kDa core protein with extensive N-linked glycosylation. A cDNA has been isolated from a human placental library using pools of oligonucleotides based on amino acid sequence obtained from purified 5T4 molecules. The predicted open reading frame encodes a protein of 420 amino acids with a molecular mass of 46 kDa and 8 potential N-glycosylation sites. There are N- and C-terminal hydrophobic segments corresponding to putative signal and membrane anchorage sequences, respectively. Northern analysis has demonstrated a major 2.5-kilobase mRNA present in cell lines serologically reactive with the monoclonal antibody 5T4. Comparison of the 5T4 protein sequence with current sequence data bases has identified the presence of leucine-rich repeats, which are found in a variety of proteins from yeast, insects, and mammals. The 5T4 antigen expression is strongly associated with metastasis in colorectal and gastric cancer, and, hence, the possible functions of the gene product and its relationship to tumor growth and progression are discussed.
dc.language.isoenen
dc.subjectCancer Antigensen
dc.subjectCancer Metastasisen
dc.subjectBiological Tumour Markersen
dc.subject.meshAmino Acid Sequence
dc.subject.meshAntigens, Neoplasm
dc.subject.meshBase Sequence
dc.subject.meshChromosome Mapping
dc.subject.meshChromosomes, Human, Pair 6
dc.subject.meshCloning, Molecular
dc.subject.meshConsensus Sequence
dc.subject.meshDNA Primers
dc.subject.meshDNA, Complementary
dc.subject.meshGene Expression
dc.subject.meshHumans
dc.subject.meshLeucine
dc.subject.meshMembrane Glycoproteins
dc.subject.meshMolecular Sequence Data
dc.subject.meshNeoplasm Metastasis
dc.subject.meshRNA, Messenger
dc.subject.meshRepetitive Sequences, Nucleic Acid
dc.subject.meshSequence Alignment
dc.subject.meshSequence Homology, Amino Acid
dc.subject.meshTrophoblasts
dc.subject.meshTumor Markers, Biological
dc.titleIsolation of a cDNA encoding 5T4 oncofetal trophoblast glycoprotein. An antigen associated with metastasis contains leucine-rich repeats.en
dc.typeArticleen
dc.contributor.departmentCancer Research Campaign Department of Immunology, Paterson Institute for Cancer Research, Christie Hospital, Manchester, United Kingdom.en
dc.identifier.journalThe Journal of Biological Chemistryen
html.description.abstractThe monoclonal antibody 5T4 defines a human oncotrophoblast antigen expressed by a variety of carcinomas but with a restricted pattern of expression in normal adult tissues. The 5T4 antigen has been isolated from term placenta as a 72-kDa glycoprotein consisting of a 42-kDa core protein with extensive N-linked glycosylation. A cDNA has been isolated from a human placental library using pools of oligonucleotides based on amino acid sequence obtained from purified 5T4 molecules. The predicted open reading frame encodes a protein of 420 amino acids with a molecular mass of 46 kDa and 8 potential N-glycosylation sites. There are N- and C-terminal hydrophobic segments corresponding to putative signal and membrane anchorage sequences, respectively. Northern analysis has demonstrated a major 2.5-kilobase mRNA present in cell lines serologically reactive with the monoclonal antibody 5T4. Comparison of the 5T4 protein sequence with current sequence data bases has identified the presence of leucine-rich repeats, which are found in a variety of proteins from yeast, insects, and mammals. The 5T4 antigen expression is strongly associated with metastasis in colorectal and gastric cancer, and, hence, the possible functions of the gene product and its relationship to tumor growth and progression are discussed.


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