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dc.contributor.authorJust, Ursula
dc.contributor.authorSpooncer, Elaine
dc.contributor.authorLöhler, J
dc.contributor.authorStocking, C
dc.contributor.authorOstertag, W
dc.contributor.authorDexter, T Michael
dc.date.accessioned2010-04-09T10:49:31Z
dc.date.available2010-04-09T10:49:31Z
dc.date.issued1994-08
dc.identifier.citationMutants of a multipotent hematopoietic cell line blocked in GM-CSF-induced differentiation are leukemogenic in vivo. 1994, 22 (9):933-40 Exp. Hematol.en
dc.identifier.issn0301-472X
dc.identifier.pmid8062891
dc.identifier.urihttp://hdl.handle.net/10541/96117
dc.description.abstractFDCP-Mix cells infected with a retroviral vector expressing the GM-CSF gene (GMV-FDCP-Mix) self-renew in the presence of interleukin-3 (IL-3), are multipotent, and undergo differentiation into granulocytes and macrophages coupled with clonal extinction after removal of IL-3. Mutants of GMV-FDCP-Mix were isolated that escape clonal extinction after differentiation induction by the autocrine secreted GM-CSF. Some of these mutant clones have a blast cell morphology and are blocked in differentiation, whereas others exhibit all stages of granulocyte and macrophage differentiation without clonal extinction. In contrast to the parental GMV-FDCP-Mix cells, all the mutants tested were leukemogenic when injected into syngeneic mice. Depending on the in vitro differentiation capacity of the transplanted mutant cell lines, the animals developed undifferentiated blast cell leukemias or CML-like syndromes. Thus, these mutant cell lines can be used to define the cooperating steps in autocrine myeloid leukemia.
dc.language.isoenen
dc.subjectCancerous Cell Transformationen
dc.subjectCancer DNAen
dc.subjectHaematopoiesisen
dc.subjectHaematopoietic Stem Cellsen
dc.subjectLeukaemiaen
dc.subject.meshAnimals
dc.subject.meshCell Differentiation
dc.subject.meshCell Line
dc.subject.meshCell Transformation, Neoplastic
dc.subject.meshDNA, Neoplasm
dc.subject.meshGene Expression Regulation
dc.subject.meshGranulocyte-Macrophage Colony-Stimulating Factor
dc.subject.meshGranulocytes
dc.subject.meshHematopoiesis
dc.subject.meshHematopoietic Stem Cells
dc.subject.meshInterleukin-3
dc.subject.meshLeukemia, Myelogenous, Chronic, BCR-ABL Positive
dc.subject.meshMacrophages
dc.subject.meshMice
dc.subject.meshMutation
dc.titleMutants of a multipotent hematopoietic cell line blocked in GM-CSF-induced differentiation are leukemogenic in vivo.en
dc.typeArticleen
dc.contributor.departmentPaterson Institute for Cancer Research, Manchester, UK.en
dc.identifier.journalExperimental Hematologyen
html.description.abstractFDCP-Mix cells infected with a retroviral vector expressing the GM-CSF gene (GMV-FDCP-Mix) self-renew in the presence of interleukin-3 (IL-3), are multipotent, and undergo differentiation into granulocytes and macrophages coupled with clonal extinction after removal of IL-3. Mutants of GMV-FDCP-Mix were isolated that escape clonal extinction after differentiation induction by the autocrine secreted GM-CSF. Some of these mutant clones have a blast cell morphology and are blocked in differentiation, whereas others exhibit all stages of granulocyte and macrophage differentiation without clonal extinction. In contrast to the parental GMV-FDCP-Mix cells, all the mutants tested were leukemogenic when injected into syngeneic mice. Depending on the in vitro differentiation capacity of the transplanted mutant cell lines, the animals developed undifferentiated blast cell leukemias or CML-like syndromes. Thus, these mutant cell lines can be used to define the cooperating steps in autocrine myeloid leukemia.


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