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    Mutants of a multipotent hematopoietic cell line blocked in GM-CSF-induced differentiation are leukemogenic in vivo.

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    Authors
    Just, Ursula
    Spooncer, Elaine
    Löhler, J
    Stocking, C
    Ostertag, W
    Dexter, T Michael
    Affiliation
    Paterson Institute for Cancer Research, Manchester, UK.
    Issue Date
    1994-08
    
    Metadata
    Show full item record
    Abstract
    FDCP-Mix cells infected with a retroviral vector expressing the GM-CSF gene (GMV-FDCP-Mix) self-renew in the presence of interleukin-3 (IL-3), are multipotent, and undergo differentiation into granulocytes and macrophages coupled with clonal extinction after removal of IL-3. Mutants of GMV-FDCP-Mix were isolated that escape clonal extinction after differentiation induction by the autocrine secreted GM-CSF. Some of these mutant clones have a blast cell morphology and are blocked in differentiation, whereas others exhibit all stages of granulocyte and macrophage differentiation without clonal extinction. In contrast to the parental GMV-FDCP-Mix cells, all the mutants tested were leukemogenic when injected into syngeneic mice. Depending on the in vitro differentiation capacity of the transplanted mutant cell lines, the animals developed undifferentiated blast cell leukemias or CML-like syndromes. Thus, these mutant cell lines can be used to define the cooperating steps in autocrine myeloid leukemia.
    Citation
    Mutants of a multipotent hematopoietic cell line blocked in GM-CSF-induced differentiation are leukemogenic in vivo. 1994, 22 (9):933-40 Exp. Hematol.
    Journal
    Experimental Hematology
    URI
    http://hdl.handle.net/10541/96117
    PubMed ID
    8062891
    Type
    Article
    Language
    en
    ISSN
    0301-472X
    Collections
    All Paterson Institute for Cancer Research

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