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dc.contributor.authorKrizanac-Bengez, L
dc.contributor.authorBoranić, M
dc.contributor.authorTesta, Nydia G
dc.contributor.authorKardum, I
dc.date.accessioned2010-04-09T10:46:06Z
dc.date.available2010-04-09T10:46:06Z
dc.date.issued1994
dc.identifier.citationNaloxone interferes with granulocytopoiesis in long-term cultures of mouse bone marrow; buffering by the stromal layer. 1994, 194 (6):375-82 Res Exp Med (Berl)en
dc.identifier.issn0300-9130
dc.identifier.pmid7716345
dc.identifier.doi10.1007/BF02576400
dc.identifier.urihttp://hdl.handle.net/10541/96116
dc.description.abstractLong-term cultures of mouse bone marrow cells were treated with naloxone, starting at the time of culture initiation or in the 2nd or 4th week of culture. Cell proliferation was suppressed and the ratio of immature and mature granulocytes to macrophages diminished by naloxone treatment. The effect depended on the timing of naloxone addition to the cultures and on its concentration, with a bell-shaped dose-response curve. High and low concentrations of naloxone (10(-4), 10(-6), 10(-14) M) interfered with hematopoiesis more strongly than the intermediate concentrations (10(-8) to 10(-12) M). Early cultures lacking the stromal layer were more sensitive to naloxone than the cultures with established stroma. The bell-shaped dose-response curve has been attributed to an interplay of specific (opioid-receptor-mediated) and nonspecific mechanisms. Opioidergic mechanisms apparently participate in the regulation of hematopoiesis.
dc.language.isoenen
dc.subjectHaematopoiesisen
dc.subject.meshAnimals
dc.subject.meshBone Marrow Cells
dc.subject.meshBuffers
dc.subject.meshCell Division
dc.subject.meshCells, Cultured
dc.subject.meshDose-Response Relationship, Drug
dc.subject.meshFemale
dc.subject.meshGranulocyte-Macrophage Colony-Stimulating Factor
dc.subject.meshGranulocytes
dc.subject.meshHematopoiesis
dc.subject.meshLeukocyte Count
dc.subject.meshLongitudinal Studies
dc.subject.meshMacrophages
dc.subject.meshMice
dc.subject.meshMice, Inbred C57BL
dc.subject.meshMice, Inbred DBA
dc.subject.meshNaloxone
dc.titleNaloxone interferes with granulocytopoiesis in long-term cultures of mouse bone marrow; buffering by the stromal layer.en
dc.typeArticleen
dc.contributor.departmentRuder Bosković Institute, Zagreb, Croatia.en
dc.identifier.journalResearch in Experimental Medicineen
html.description.abstractLong-term cultures of mouse bone marrow cells were treated with naloxone, starting at the time of culture initiation or in the 2nd or 4th week of culture. Cell proliferation was suppressed and the ratio of immature and mature granulocytes to macrophages diminished by naloxone treatment. The effect depended on the timing of naloxone addition to the cultures and on its concentration, with a bell-shaped dose-response curve. High and low concentrations of naloxone (10(-4), 10(-6), 10(-14) M) interfered with hematopoiesis more strongly than the intermediate concentrations (10(-8) to 10(-12) M). Early cultures lacking the stromal layer were more sensitive to naloxone than the cultures with established stroma. The bell-shaped dose-response curve has been attributed to an interplay of specific (opioid-receptor-mediated) and nonspecific mechanisms. Opioidergic mechanisms apparently participate in the regulation of hematopoiesis.


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