O6-alkylguanine-DNA alkyltransferase activity in schistosomiasis-associated human bladder cancer.
Authors
Badawi, A FCooper, Donald P
Mostafa, M H
Aboul-Azm, T
Barnard, R J
Margison, Geoffrey P
O'Connor, Peter J
Affiliation
National Center for Toxicology, Jefferson, Arkansas.Issue Date
1994
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Show full item recordAbstract
O6-Alkylguanine-DNA-alkyltransferase (ATase) activity was measured in extracts of 55 bladder tissue samples (46 tumour and nine uninvolved mucosal tissue) from Egyptian patients with schistosome-associated bladder carcinoma. Activity varied from 2.0 to 16.2 fmole ATase/microgram DNA (mean +/- S.D.; 5.6 +/- 4.0) or from 28 to 351 fmole ATase/mg (117 +/- 71). ATase levels in schistosome-associated bladder cancer tissues (5.6 +/- 4.0 fmole ATase/microgram DNA) tended to be lower than those observed in normal human bladder mucosal tissue (8.5 +/- 4.4 fmole ATase/microgram DNA). In a previous study (Badawi et al., Carcinogenesis, 1992, 13, 877-881) DNA-alkylation damage (O6-methyldeoxyguanosine) was found in 44/46 of these schistosome-associated bladder cancer samples at levels ranging from 0.012 to 0.485 mumole O6-MedG/mole deoxyguanosine. We now report an inverse correlation between the levels of methylation damage and ATase activity (r = -0.67; P < 0.001). These observations encourage further investigations of the possible role of environmental alkylating agents in the aetiology of early bladder cancer associated with schistosomiasis.Citation
O6-alkylguanine-DNA alkyltransferase activity in schistosomiasis-associated human bladder cancer. 1994, 30A (9):1314-9 Eur. J. CancerJournal
European Journal of CancerDOI
10.1016/0959-8049(94)90180-5PubMed ID
7999420Type
ArticleLanguage
enISSN
0959-8049ae974a485f413a2113503eed53cd6c53
10.1016/0959-8049(94)90180-5
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