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    Association of thrombospondin-1 with osteogenic differentiation of retinal pericytes in vitro.

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    Authors
    Canfield, A E
    Sutton, Andrew B
    Hoyland, J A
    Schor, Ana M
    Affiliation
    University of Manchester, School of Biological Sciences, UK.
    Issue Date
    1996-02
    
    Metadata
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    Abstract
    Vascular pericytes can differentiate into osteoblast-like cells in vitro, suggesting that these cells may represent a potential source of osteoprogenitor cells in the adult. Pericyte differentiation is associated with a characteristic pattern of nodule formation and mineralisation. Nodules are formed in post-confluent cultures by the retraction of multilayered areas. Crystals of hydroxyapatite are deposited on the extracellular matrix of these nodules which then becomes mineralised. We now demonstrate that thrombospondin-1 (TSP-1) gene expression is modulated during pericyte differentiation in vitro. That is, the relative levels of TSP-1 (protein and mRNA) increased markedly during nodule formation and then decreased when mineralisation of the nodules had taken place. TSP-1 was localised throughout non-mineralised nodules but it was largely excluded from the inner mass of mineralised nodules. The production of a mineralised matrix by vascular pericytes was promoted by the presence of antibodies to TSP-1 in the culture medium and was inhibited by exogenous TSP-1. These effects did not appear to be mediated through the activation of latent TGF-beta, since neither exogenous TGF-beta nor neutralising antibodies to TGF-beta had any effect on the rate or extent of mineralisation seen in the pericyte cultures. Taken together these results suggest that high levels of TSP-1 inhibit pericyte mineralisation, supporting the view that this protein plays a role in pericyte differentiation and bone formation.
    Citation
    Association of thrombospondin-1 with osteogenic differentiation of retinal pericytes in vitro. 1996, 109 ( Pt 2):343-53 J. Cell. Sci.
    Journal
    Journal of Cell Science
    URI
    http://hdl.handle.net/10541/96007
    PubMed ID
    8838658
    Type
    Article
    Language
    en
    ISSN
    0021-9533
    Collections
    All Christie Publications

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