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    Nuclear pore clustering is a consistent feature of apoptosis in vitro.

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    Authors
    Reipert, Siegfried
    Reipert, Brigit M
    Hickman, John A
    Allen, Terence D
    Affiliation
    CRC, Department of Structural Cell Biology, Paterson Institute for Cancer Research and Christie Hospital, Manchester, UK.
    Issue Date
    1996-01
    
    Metadata
    Show full item record
    Abstract
    Two cell lines which show different patterns of DNA fragmentation have been examined for variations of their nuclear morphology during apoptosis. FDCP-Mix, a pluripotent murine haemopoietic stem cell line which undergoes typical internucleosomal cleavage of DNA when induced to apoptosis either by drugs or withdrawal of growth factor (IL-3) was compared with the human lymphoid leukemia cell line MOLT-4, a cell line which undergoes apoptosis without production of a typical DNA 'ladder'. The nuclear morphology of FDCP-Mix cells was consistent after apoptotic induction by drug or by growth factor withdrawal. Apoptotic nuclear morphology for MOLT-4 and FDCP-Mix showed variations in the distribution, density and texture of the electron dense nuclear marginations. Despite these differences, clustering of nuclear pore complexes (NPCs) after treatment with the topoisomerase II inhibitor etoposide was a common phenomenon for both cell lines. Moreover, pore clustering for FDCP-Mix nuclei occurred independently from the way in which apoptosis was induced, either by growth factor withdrawal or etoposide treatment. In a novel approach, we visualised the clustering of NPCs three-dimensionally by field emission in-lens scanning electron microscopy (FEISEM).
    Citation
    Nuclear pore clustering is a consistent feature of apoptosis in vitro. 1996, 3 (1):131-9 Cell Death Differ.
    Journal
    Cell Death and Differentiation
    URI
    http://hdl.handle.net/10541/95877
    PubMed ID
    17180065
    Type
    Article
    Language
    en
    ISSN
    1350-9047
    Collections
    All Paterson Institute for Cancer Research

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