Response of V79 cells to low doses of X-rays and negative pi-mesons: clonogenic survival and DNA strand breaks.
AffiliationMedical Biophysics, BC Cancer Research Centre, Vancouver, Canada.
MetadataShow full item record
AbstractMammalian cells are hypersensitive to very low doses of X-rays (< 0.2 Gy), a response which is followed by increased radioresistance up to 1 Gy. Increased radioresistance is postulated to be a response to DNA damage, possibly single-strand breaks, and it appears to be a characteristic of low linear energy transfer (LET) radiation. Here we demonstrate a correspondence between the extent of the increased radioresistance and linear energy transfer of 250 kVp X-rays and plateau and Bragg peak negative pi-mesons. The results support our hypothesis since the size of the increased radioresistant response appears to correspond to the number of radiation induced single-strand breaks. Furthermore, since survival prior to the increased radioresistant response (< 0.2 Gy) was LET-independent, these data support the notion that the increased radioresistant response may dictate the overall survival response to higher doses. However, while these data provide further circumstantial evidence for the involvement of DNA strand breaks in the triggering of increased radioresistance, more direct conclusions cannot be made. The data are not accurate enough to detect structure in the single-strand break profiles, the production of single-strand breaks being apparently linear with dose.
CitationResponse of V79 cells to low doses of X-rays and negative pi-mesons: clonogenic survival and DNA strand breaks. 1996, 70 (4):429-36 Int. J. Radiat. Biol.
JournalInternational Journal of Radiation Biology
- Small doses of high-linear energy transfer radiation increase the radioresistance of Chinese hamster V79 cells to subsequent X irradiation.
- Authors: Marples B, Skov KA
- Issue date: 1996 Oct
- The response of Chinese hamster V79-379A cells exposed to negative pi-mesons: evidence that increased radioresistance is dependent on linear energy transfer.
- Authors: Marples B, Lam GK, Zhou H, Skov KA
- Issue date: 1994 Apr
- Negative pion irradiation of mammalian cells. III. A comparative analysis of DNA strand breakage, repair and cell survival after exposure to pi-mesons and X-rays.
- Authors: Weibezahn KF, Sexauer C, Coquerelle T
- Issue date: 1980 Oct
- Induction and rejoining of DNA double-strand breaks in Chinese hamster V79-4 cells irradiated with characteristic aluminum K and copper L ultrasoft X rays.
- Authors: Botchway SW, Stevens DL, Hill MA, Jenner TJ, O'Neill P
- Issue date: 1997 Oct