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Mutation of the TP53 gene and allelic imbalance at chromosome 17p13 in ductal carcinoma in situ.

dc.contributor.authorMunn, K E
dc.contributor.authorWalker, R A
dc.contributor.authorMenasce, Lia P
dc.contributor.authorVarley, Jennifer
dc.date.accessioned2010-04-07T10:43:23Z
dc.date.available2010-04-07T10:43:23Z
dc.date.issued1996-11
dc.identifier.citationMutation of the TP53 gene and allelic imbalance at chromosome 17p13 in ductal carcinoma in situ. 1996, 74 (10):1578-85 Br. J. Canceren
dc.identifier.issn0007-0920
dc.identifier.pmid8932338
dc.identifier.urihttp://hdl.handle.net/10541/95827
dc.description.abstractA panel of 36 cases of preinvasive breast lesions, including 35 cases of ductal carcinoma in situ (DCIS), has been examined for mutation of TP53, allelic imbalance (AI) on 17p13, and expression of TP53, in a number of cases, has been studied using immunohistochemistry. Areas of DCIS, with or without adjacent invasive or benign cells, have been separately microdissected from paraffin-embedded sections and analysed by PCR for genetic changes to chromosome 17p13. TP53 mutations and AI on 17p have been identified in cases of 'pure' DCIS as well as those with associated invasive carcinoma and, furthermore, have been identified in well-differentiated lesions as well as poorly differentiated ones.
dc.language.isoenen
dc.subjectBreast Canceren
dc.subjectCancer Stagingen
dc.subject.meshAlleles
dc.subject.meshBreast Neoplasms
dc.subject.meshCarcinoma in Situ
dc.subject.meshCarcinoma, Ductal, Breast
dc.subject.meshChromosomes, Human, Pair 17
dc.subject.meshDisease Progression
dc.subject.meshFemale
dc.subject.meshGene Expression
dc.subject.meshGenes, p53
dc.subject.meshHumans
dc.subject.meshImmunohistochemistry
dc.subject.meshMutation
dc.subject.meshNeoplasm Staging
dc.titleMutation of the TP53 gene and allelic imbalance at chromosome 17p13 in ductal carcinoma in situ.en
dc.typeArticleen
dc.contributor.departmentCRC Department of Cancer Genetics, Paterson Institute for Cancer Research, Christie Hospital, Manchester, UK.en
dc.identifier.journalBritish Journal of Canceren
html.description.abstractA panel of 36 cases of preinvasive breast lesions, including 35 cases of ductal carcinoma in situ (DCIS), has been examined for mutation of TP53, allelic imbalance (AI) on 17p13, and expression of TP53, in a number of cases, has been studied using immunohistochemistry. Areas of DCIS, with or without adjacent invasive or benign cells, have been separately microdissected from paraffin-embedded sections and analysed by PCR for genetic changes to chromosome 17p13. TP53 mutations and AI on 17p have been identified in cases of 'pure' DCIS as well as those with associated invasive carcinoma and, furthermore, have been identified in well-differentiated lesions as well as poorly differentiated ones.


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