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dc.contributor.authorIshiguro, A
dc.contributor.authorSpirin, K S
dc.contributor.authorShiohara, M
dc.contributor.authorTobler, A
dc.contributor.authorGombart, A F
dc.contributor.authorIsrael, M A
dc.contributor.authorNorton, John D
dc.contributor.authorKoeffler, H P
dc.date.accessioned2010-04-07T10:06:32Z
dc.date.available2010-04-07T10:06:32Z
dc.date.issued1996-06-15
dc.identifier.citationId2 expression increases with differentiation of human myeloid cells. 1996, 87 (12):5225-31 Blooden
dc.identifier.issn0006-4971
dc.identifier.pmid8652837
dc.identifier.urihttp://hdl.handle.net/10541/95824
dc.description.abstractId proteins are helix-loop-helix (HLH) transcriptional factors that lack the basic DNA binding domain. The Id proteins have been reported generally to function as inhibitors of cell differentiation, and their gene expression is often downregulated during cell differentiation. We examined the expression of human Id mRNAs by Northern hybridization in 11 human myeloid cell lines, several myeloid cell lines induced to differentiate, fresh myeloid leukemia samples, and normal human myeloid cells. Id2 mRNA was expressed in myelomonoblastic and monoblastic leukemic cells (PLB-985, THP-1, and U-937) but was weakly expressed in myeloblastic leukemic cells (KG-1 and HL-60). Id2 mRNA levels markedly increased with induction of differentiation of myeloid blasts (HL-60, PLB-985, THP-1, and U-937) toward either granulocytes or macrophages. Examination of fresh acute myeloid leukemic samples from 22 individuals also showed prominent Id2 mRNA expression in those samples having more differentiated blasts. Using the French-American-British classification, only 2 of 8 M0/M1 samples expressed Id2 mRNA; however, 10 of 13 M2/M3/M4 samples expressed it. In normal human myeloid cells, Id2 mRNA was expressed in cultured macrophages from bone marrow and in mature granulocytes and monocytes from peripheral blood. The half-life of Id2 mRNA was short (1 hour), and its expression was inducible by cessation of protein synthesis. Id3 mRNA was moderately expressed in monoblastic cell lines (THP-1 and U-937), and levels decreased with their differentiation. Almost no Id3 expression was detectable in either other myeloid leukemia lines, fresh leukemic samples, or normal human myeloid cells by Northern analyses. Id1 mRNA was not detected by polymerase chain reaction in either leukemic or normal myeloid cells except in K562 myeloid/erythroid cells. These results showed that Id2 mRNA was constitutively expressed in more mature myeloid blast cells and level markedly increased with terminal myeloid differentiation, suggesting that Id2 protein may inhibit an HLH transcriptional complex that normally represses myeloid differentiation.
dc.language.isoenen
dc.subjectLeukaemic Gene Expression Regulationen
dc.subjectAcute Monocytic Leukaemiaen
dc.subjectMyeloid Leukaemiaen
dc.subjectCancerous Stem Cellsen
dc.subjectCancer RNAen
dc.subjectCultured Tumour Cellsen
dc.subject.meshAcute Disease
dc.subject.meshBase Sequence
dc.subject.meshCell Differentiation
dc.subject.meshDNA-Binding Proteins
dc.subject.meshGene Expression Regulation
dc.subject.meshGene Expression Regulation, Leukemic
dc.subject.meshHL-60 Cells
dc.subject.meshHematopoietic Stem Cells
dc.subject.meshHumans
dc.subject.meshInhibitor of Differentiation Protein 2
dc.subject.meshLeukemia, Monocytic, Acute
dc.subject.meshLeukemia, Myeloid
dc.subject.meshLymphoma, Large B-Cell, Diffuse
dc.subject.meshMolecular Sequence Data
dc.subject.meshNeoplasm Proteins
dc.subject.meshNeoplastic Stem Cells
dc.subject.meshPolymerase Chain Reaction
dc.subject.meshRNA, Messenger
dc.subject.meshRNA, Neoplasm
dc.subject.meshRepressor Proteins
dc.subject.meshTranscription Factors
dc.subject.meshTumor Cells, Cultured
dc.titleId2 expression increases with differentiation of human myeloid cells.en
dc.typeArticleen
dc.contributor.departmentDepartment of Medicine, Cedars-Sinai Medical Center, UCLA School of Medicine, Los Angeles, CA 90048, USA.en
dc.identifier.journalBlooden
html.description.abstractId proteins are helix-loop-helix (HLH) transcriptional factors that lack the basic DNA binding domain. The Id proteins have been reported generally to function as inhibitors of cell differentiation, and their gene expression is often downregulated during cell differentiation. We examined the expression of human Id mRNAs by Northern hybridization in 11 human myeloid cell lines, several myeloid cell lines induced to differentiate, fresh myeloid leukemia samples, and normal human myeloid cells. Id2 mRNA was expressed in myelomonoblastic and monoblastic leukemic cells (PLB-985, THP-1, and U-937) but was weakly expressed in myeloblastic leukemic cells (KG-1 and HL-60). Id2 mRNA levels markedly increased with induction of differentiation of myeloid blasts (HL-60, PLB-985, THP-1, and U-937) toward either granulocytes or macrophages. Examination of fresh acute myeloid leukemic samples from 22 individuals also showed prominent Id2 mRNA expression in those samples having more differentiated blasts. Using the French-American-British classification, only 2 of 8 M0/M1 samples expressed Id2 mRNA; however, 10 of 13 M2/M3/M4 samples expressed it. In normal human myeloid cells, Id2 mRNA was expressed in cultured macrophages from bone marrow and in mature granulocytes and monocytes from peripheral blood. The half-life of Id2 mRNA was short (1 hour), and its expression was inducible by cessation of protein synthesis. Id3 mRNA was moderately expressed in monoblastic cell lines (THP-1 and U-937), and levels decreased with their differentiation. Almost no Id3 expression was detectable in either other myeloid leukemia lines, fresh leukemic samples, or normal human myeloid cells by Northern analyses. Id1 mRNA was not detected by polymerase chain reaction in either leukemic or normal myeloid cells except in K562 myeloid/erythroid cells. These results showed that Id2 mRNA was constitutively expressed in more mature myeloid blast cells and level markedly increased with terminal myeloid differentiation, suggesting that Id2 protein may inhibit an HLH transcriptional complex that normally represses myeloid differentiation.


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