Chromosomal radiosensitivity in G2-phase lymphocytes as an indicator of cancer predisposition.
AffiliationPaterson Institute for Cancer Research, Christie CRC Research Centre, Manchester, United Kingdom.
MetadataShow full item record
AbstractSanford et al. (Int. J. Radiat. Biol. 55, 963-981, 1989) have reported that G2-phase cells from many heritable cancer-prone conditions exhibit higher yields of X-ray-induced chromosome damage than those found in the majority of healthy controls. We have applied their protocol to lymphocytes of a group of control and cancer-prone individuals to see if we could confirm these observations. For control donors we observed higher aberration yields, different kinetics and more interexperiment variability than found by Sanford et al. These differences could not be attributed to unavoidable minor variations in procedures (e.g. serum batches, glassware washing methods), but the difference in X-ray qualities used in the two laboratories may have made a small contribution to the discrepancies. We attribute some of our experimental variability to the fact that, to varying extents in different experiments, centrifugation of cells prior to irradiation can slow down the progression of cells into metaphase and that cells can continue to repair during the harvesting procedure (centrifugation and hypotonic treatment). We have applied the assay to cases of ataxia telangiectasia (AT, homozygotes and heterozygotes), xeroderma pigmentosum (homozygotes and heterozygotes), familial adenomatous polyposis and the syndromes Li-Fraumeni, basal cell nevus, Down's and Fanconi's but have been unable to discriminate between these groups and controls except for AT homozygotes. By including a control sample in parallel with samples from cancer-prone groups we found a significant difference in mean aberration yields between controls and AT homozygotes and heterozygotes, but not for the other groups. Since technical features could explain the discrepancies between our laboratories, we have devised our own G2-phase assay which appears to be giving promising results.
CitationChromosomal radiosensitivity in G2-phase lymphocytes as an indicator of cancer predisposition. 1996, 145 (1):3-16 Radiat. Res.
- G2 radiosensitivity of cells derived from cancer-prone individuals.
- Authors: Darroudi F, Vyas RC, Vermeulen S, Natarajan AT
- Issue date: 1995 Apr
- G2 chromosomal radiosensitivity in fibroblasts of ataxia-telangiectasia heterozygotes and a Li-Fraumeni syndrome patient with radioresistant cells.
- Authors: Mitchell EL, Scott D
- Issue date: 1997 Oct
- Chromosomal radiosensitivity, cancer predisposition and response to radiotherapy.
- Authors: Scott D
- Issue date: 2000 May
- Radioprotective effect of amifostine on cells from cancer prone patients and healthy individuals studied by the G2 and PCC assays.
- Authors: Manola KN, Terzoudi GI, Dardoufas CE, Malik SI, Pantelias GE
- Issue date: 2003 Oct
- Chromosomal radiosensitivity during the G2 cell-cycle period of skin fibroblasts from individuals with familial cancer.
- Authors: Parshad R, Sanford KK, Jones GM
- Issue date: 1985 Aug