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dc.contributor.authorWatson, A J
dc.contributor.authorMerritt, A J
dc.contributor.authorJones, L S
dc.contributor.authorAskew, J N
dc.contributor.authorAnderson, Elizabeth
dc.contributor.authorBecciolini, A
dc.contributor.authorBalzi, M
dc.contributor.authorPotten, Christopher S
dc.contributor.authorHickman, John A
dc.date.accessioned2010-04-06T10:41:07Z
dc.date.available2010-04-06T10:41:07Z
dc.date.issued1996-04
dc.identifier.citationEvidence for reciprocity of bcl-2 and p53 expression in human colorectal adenomas and carcinomas. 1996, 73 (8):889-95 Br. J. Canceren
dc.identifier.issn0007-0920
dc.identifier.pmid8611422
dc.identifier.urihttp://hdl.handle.net/10541/95657
dc.description.abstractEvidence of accumulating for the failure of apoptosis as an important factor in the evolution of colorectal cancer and its poor response to adjuvant therapy. The proto-oncogene bcl-2 suppresses apoptosis. Its expression could provide an important survival advantage permitting the development of colorectal cancer. The expression of bcl-2 and p53 was determined by immunohistochemistry in 47 samples of histologically normal colonic mucosa, 19 adenomas and 53 adenocarcinomas. Expression of bcl-2 in colonic crypts > 5 cm from the tumours was confined to crypt bases but was more extensive and intense in normal crypts < 5 mm from cancers. A higher proportion of adenomas (63.2%) than carcinomas (36.5%) expressed bcl-2 (P < 0.05). A lower proportion of adenomas (31.6%) than carcinomas (62.3%) expressed p53 (P < 0.02). A total of 26.3% of adenomas and 22% of carcinomas expressed both bcl-2 and p53. To determine whether these samples contained cells which expressed both proteins, a dual staining technique for bcl-2 and p53 was used. Only 1/19 adenomas and 2/53 carcinomas contained cells immunopositive for both bcl-2 and p53. Moreover there was evidence of reciprocity of expression of bcl-2 and p53 in these three double staining neoplasms. We suggest that bcl-2 provides a survival advantage in the proliferative compartment of normal crypts and colorectal neoplasms. However, its expression is lost during the evolution from adenoma to carcinoma, whereas p53 expression is increased, an event generally coincident with the expression of stabilised p53, which we presume to represent the mutant form.
dc.language.isoenen
dc.subjectColorectal Canceren
dc.subjectTumour Suppressor Protein p53en
dc.subject.meshAdenocarcinoma
dc.subject.meshAdenoma
dc.subject.meshColorectal Neoplasms
dc.subject.meshHumans
dc.subject.meshImmunohistochemistry
dc.subject.meshProto-Oncogene Proteins
dc.subject.meshProto-Oncogene Proteins c-bcl-2
dc.subject.meshTumor Suppressor Protein p53
dc.titleEvidence for reciprocity of bcl-2 and p53 expression in human colorectal adenomas and carcinomas.en
dc.typeArticleen
dc.contributor.departmentDepartment of Medicine, University of Manchester, Hope Hospital, Salford, UK.en
dc.identifier.journalBritish Journal of Canceren
html.description.abstractEvidence of accumulating for the failure of apoptosis as an important factor in the evolution of colorectal cancer and its poor response to adjuvant therapy. The proto-oncogene bcl-2 suppresses apoptosis. Its expression could provide an important survival advantage permitting the development of colorectal cancer. The expression of bcl-2 and p53 was determined by immunohistochemistry in 47 samples of histologically normal colonic mucosa, 19 adenomas and 53 adenocarcinomas. Expression of bcl-2 in colonic crypts > 5 cm from the tumours was confined to crypt bases but was more extensive and intense in normal crypts < 5 mm from cancers. A higher proportion of adenomas (63.2%) than carcinomas (36.5%) expressed bcl-2 (P < 0.05). A lower proportion of adenomas (31.6%) than carcinomas (62.3%) expressed p53 (P < 0.02). A total of 26.3% of adenomas and 22% of carcinomas expressed both bcl-2 and p53. To determine whether these samples contained cells which expressed both proteins, a dual staining technique for bcl-2 and p53 was used. Only 1/19 adenomas and 2/53 carcinomas contained cells immunopositive for both bcl-2 and p53. Moreover there was evidence of reciprocity of expression of bcl-2 and p53 in these three double staining neoplasms. We suggest that bcl-2 provides a survival advantage in the proliferative compartment of normal crypts and colorectal neoplasms. However, its expression is lost during the evolution from adenoma to carcinoma, whereas p53 expression is increased, an event generally coincident with the expression of stabilised p53, which we presume to represent the mutant form.


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