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    The quantitation and kinetics of unscheduled (repair) DNA synthesis in ultraviolet-irradiated human skin by automated image analysis.

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    Authors
    Chadwick, Caroline A
    Diggle, S P
    Young, A R
    Potten, Christopher S
    Affiliation
    CRC Department of Epithelial Biology, Paterson Institute for Cancer Research, Christie Hospital NHS Trust, Manchester, U.K.
    Issue Date
    1996-10
    
    Metadata
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    Abstract
    Grain counting by eye is a tedious and time-consuming technique but one with great potential in cell kinetics and for the study of DNA excision repair activity (unscheduled DNA synthesis or UDS). We have been investigating the levels of UDS in human skin sections exposed in situ to ultraviolet radiation using a short-term incubation in tritiated thymidine and autoradiography and the decline in UDS levels with time (repair kinetics). We have adapted an automated image analysis system automatically to assess the number of grains over epidermal cell nuclei in autoradiographs of sections of epidermis. An excellent correlation was observed between visual counting and machine measurement of the area (in pixels) occupied by silver grains. The levels of UDS declined with time as lesions are progressively repaired. The half time (+/- standard deviation) for the reduction in UDS is 7.25 +/- 0.18 h. The grain counts can be significantly increased by increasing the autoradiographic exposure, by increasing the concentration of tritiated thymidine and by increasing the incubation time.
    Citation
    The quantitation and kinetics of unscheduled (repair) DNA synthesis in ultraviolet-irradiated human skin by automated image analysis. 1996, 135 (4):516-22 Br. J. Dermatol.
    Journal
    The British Journal of Dermatology
    URI
    http://hdl.handle.net/10541/95624
    DOI
    10.1046/j.1365-2133.1996.d01-1032.x
    PubMed ID
    8915138
    Type
    Article
    Language
    en
    ISSN
    0007-0963
    ae974a485f413a2113503eed53cd6c53
    10.1046/j.1365-2133.1996.d01-1032.x
    Scopus Count
    Collections
    All Paterson Institute for Cancer Research

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