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    Priming in vivo and quantification in vitro of class I MHC-restricted cytotoxic T cells to human papilloma virus type 11 early proteins (E6 and E7) using immunostimulating complexes (ISCOMs).

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    Authors
    Tarpey, I
    Stacey, S N
    McIndoe, A
    Davies, D H
    Affiliation
    Division of Life Sciences, King's College, London, UK.
    Issue Date
    1996-02
    
    Metadata
    Show full item record
    Abstract
    Immunostimulating complexes (ISCOMs) efficiently deliver soluble antigen into both the cytosolic (endogenous) and endosomal (exogenous) pathways of antigen processing. Cytosolic delivery to antigen-presenting cells (APCs) may therefore be useful for the stimulation and assay of class I major histocompatibility complex (MHC)-restricted cytotoxic T lymphocytes (CTL) in vitro. In this study, mice were immunized with ISCOMs containing fusion proteins of the E6 or E7 early proteins of human papilloma virus type 11 (HPV 11) to elicit CTL. These CTL were then restimulated in vitro using APCs pulsed with the same ISCOMs, prior to cytotoxicity assay using syngeneic target cells infected with recombinant vaccinia viruses. In this way, antigen-specific, MHC-restricted lysis by CD8+ cells was detected. However, this was dependent on the use of low density splenocytes as APCs for restimulation in vitro. Limiting dilution analyses showed a direct correlation between the CTL responder frequency and the number of times the animals were immunized in vivo. We conclude that in lieu of infectious virus, the use of ISCOMs to mediate antigen delivery to APCs in vitro can be used to quantitate CTL activity. This may have applications in monitoring vaccine efficacy, particularly to viruses such as HPV, which cannot be presently obtained as infectious virus in sufficient quantity for CTL propagation and assay.
    Citation
    Priming in vivo and quantification in vitro of class I MHC-restricted cytotoxic T cells to human papilloma virus type 11 early proteins (E6 and E7) using immunostimulating complexes (ISCOMs). 1996, 14 (3):230-6 Vaccine
    Journal
    Vaccine
    URI
    http://hdl.handle.net/10541/95623
    DOI
    10.1016/0264-410X(95)00179-5
    PubMed ID
    8920705
    Type
    Article
    Language
    en
    ISSN
    0264-410X
    ae974a485f413a2113503eed53cd6c53
    10.1016/0264-410X(95)00179-5
    Scopus Count
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    All Paterson Institute for Cancer Research

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