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dc.contributor.authorHelftenbein, G
dc.contributor.authorKrusekopf, K
dc.contributor.authorJust, U
dc.contributor.authorCross, M
dc.contributor.authorOstertag, W
dc.contributor.authorNiemann, H
dc.contributor.authorTamura, T
dc.date.accessioned2010-04-06T09:23:00Z
dc.date.available2010-04-06T09:23:00Z
dc.date.issued1996-02-15
dc.identifier.citationTranscriptional regulation of the c-fms proto-oncogene mediated by granulocyte/macrophage colony-stimulating factor (GM-CSF) in murine cell lines. 1996, 12 (4):931-5 Oncogeneen
dc.identifier.issn0950-9232
dc.identifier.pmid8632916
dc.identifier.urihttp://hdl.handle.net/10541/95622
dc.description.abstractDifferentiation of blood cells is paralleled by a timely ordered expression of cytokine receptor genes. We show here that the expression of the c-fms gene which encodes the lineage-specific receptor for macrophage colony-stimulating factor (M-CSF or CSF-1) is directly linked to ligand-mediated activation of the receptor for the granulocyte/macrophage colony-stimulating factor (GM-CSF). In interleukin-3 (IL-3) dependent multipotent progenitor cells, FDC-Pmix GMV#2 cells, GM-CSF treatment results in the rapid formation of full-length c-fms transcripts. Surprisingly, this upregulation of c-fms transcripts is also observed in mouse NIH3T3 fibroblasts stably transfected with genes coding for the alpha- and beta-subunits of the GM-CSF receptor. These results indicate a direct control by the GM-CSF receptor that takes place regardless of cell differentiation. Furthermore, a 2.1 kb genomic fragment containing the c-fms proximal promoter directs GM-CSF-inducible expression of a reporter gene, suggesting a regulation of c-fms gene expression on the transcriptional level.
dc.language.isoenen
dc.subjectHaematopoietic Stem Cellsen
dc.subject.mesh3T3 Cells
dc.subject.meshAnimals
dc.subject.meshCell Differentiation
dc.subject.meshCell Line
dc.subject.meshGene Expression Regulation
dc.subject.meshGenes, fms
dc.subject.meshGranulocyte-Macrophage Colony-Stimulating Factor
dc.subject.meshGranulocytes
dc.subject.meshHematopoietic Stem Cells
dc.subject.meshInterleukin-3
dc.subject.meshKinetics
dc.subject.meshMacrophages
dc.subject.meshMice
dc.subject.meshPromoter Regions, Genetic
dc.subject.meshReceptor, Macrophage Colony-Stimulating Factor
dc.subject.meshReceptors, Granulocyte-Macrophage Colony-Stimulating Factor
dc.subject.meshRecombinant Proteins
dc.subject.meshTranscription, Genetic
dc.subject.meshTransfection
dc.titleTranscriptional regulation of the c-fms proto-oncogene mediated by granulocyte/macrophage colony-stimulating factor (GM-CSF) in murine cell lines.en
dc.typeArticleen
dc.contributor.departmentInstitut für Virologie, Justus-Liebig Universität Giessen, Germany.en
dc.identifier.journalOncogeneen
html.description.abstractDifferentiation of blood cells is paralleled by a timely ordered expression of cytokine receptor genes. We show here that the expression of the c-fms gene which encodes the lineage-specific receptor for macrophage colony-stimulating factor (M-CSF or CSF-1) is directly linked to ligand-mediated activation of the receptor for the granulocyte/macrophage colony-stimulating factor (GM-CSF). In interleukin-3 (IL-3) dependent multipotent progenitor cells, FDC-Pmix GMV#2 cells, GM-CSF treatment results in the rapid formation of full-length c-fms transcripts. Surprisingly, this upregulation of c-fms transcripts is also observed in mouse NIH3T3 fibroblasts stably transfected with genes coding for the alpha- and beta-subunits of the GM-CSF receptor. These results indicate a direct control by the GM-CSF receptor that takes place regardless of cell differentiation. Furthermore, a 2.1 kb genomic fragment containing the c-fms proximal promoter directs GM-CSF-inducible expression of a reporter gene, suggesting a regulation of c-fms gene expression on the transcriptional level.


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