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    The nuclear pore complex and lamina: three-dimensional structures and interactions determined by field emission in-lens scanning electron microscopy.

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    Authors
    Goldberg, Martin W
    Allen, Terence D
    Affiliation
    CRC Department of Structural Cell Biology, Paterson Institute for Cancer Research, Christie Hospital NHS Trust, UK.
    Issue Date
    1996-04-12
    
    Metadata
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    Abstract
    Three dimensional surface imaging was used to examine structural details of the nuclear pore complex. Subsurface structures were uncovered by detergent extraction, proteolysis, mechanical fracturing and combinations of these. Features observed in this way were mostly consistent with previous three-dimensional structures with some novel observations. In addition to cytoplasmic and basket filaments attached to each coaxial ring, we have observed radiating filaments within the central channel. New details of basket organization are presented, showing that basket filaments branch and are woven together to form the basket ring. The "central transporter" is shown to be a regular, consistent structure revealed after removal of overlying internal filaments. The lumenal ring is visualised and we present evidence that the lamina may be attached to the spoke ring complex. Finally we show that there is evidence for a novel structure, the "star ring", sandwiched between the cytoplasmic ring and the membrane.
    Citation
    The nuclear pore complex and lamina: three-dimensional structures and interactions determined by field emission in-lens scanning electron microscopy. 1996, 257 (4):848-65 J. Mol. Biol.
    Journal
    Journal of Molecular Biology
    URI
    http://hdl.handle.net/10541/95610
    DOI
    10.1006/jmbi.1996.0206
    PubMed ID
    8636986
    Type
    Article
    Language
    en
    ISSN
    0022-2836
    ae974a485f413a2113503eed53cd6c53
    10.1006/jmbi.1996.0206
    Scopus Count
    Collections
    All Paterson Institute for Cancer Research

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