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    A comparison of proliferation markers (BrdUrd, Ki-67, PCNA) determined at each cell position in the crypts of normal human colonic mucosa.

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    Authors
    Bromley, Michael
    Rew, D
    Becciolini, Aldo
    Balzi, Manuela
    Chadwick, Caroline A
    Hewitt, D
    Li, Y
    Potten, Christopher S
    Affiliation
    CRC Department of Histology, Paterson Institute for Cancer Research, Christie Hospital (NHS) Trust, Manchester, UK.
    Issue Date
    1996
    
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    Abstract
    Samples of microscopically normal human sigmoid colon fixed in 70% ethanol from 15 patients who had received bromodeoxyuridine (BrdUrd) prior to surgery have been reanalyzed using a combination of proliferation markers. The specimens have been immunostained for proliferating cell nuclear antigen (PCNA) and after microwave treatment, they have been stained for BrdUrd and Ki-67. The 15 patients selected comprised 5 patients whose mucosa previously gave high BrdUrd labelling indices in the crypt, 5 that gave median values for BrdUrd labelling and 5 that gave low values for bromodeoxyuridine labelling on a previous analysis using tissue fixed in 70% ethanol and formal saline and using a different antibody (Potten et al., 1992). The relative levels of labelling at each cell position in the crypts has been compared using the 3 proliferation markers with the data being compared with the BrdUrd labelling as a standard labelling for S phase cells. One objective was to see whether all three proliferation markers discriminated equally well between the three groups of patient samples. The data show that the distinction between high, medium and low values seen with BrdUrd labelling was retained when Ki-67 immunostaining was analysed. PCNA immunostaining resulted in high levels of labelling and the different levels of labelling seen with BrdUrd and Ki-67 were largely lost.
    Citation
    A comparison of proliferation markers (BrdUrd, Ki-67, PCNA) determined at each cell position in the crypts of normal human colonic mucosa. 1996, 40 (2):89-100 Eur J Histochem
    Journal
    European Journal of Histochemistry
    URI
    http://hdl.handle.net/10541/95530
    PubMed ID
    8839702
    Type
    Article
    Language
    en
    ISSN
    1121-760X
    Collections
    All Paterson Institute for Cancer Research

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