Accessing nuclear structure for field emission, in lens, scanning electron microscopy (FEISEM).
AuthorsAllen, Terence D
Bennion, G R
Rutherford, Sandra A
Goldberg, Martin W
AffiliationCRC Department of Structural Cell Biology, Paterson Institute for Cancer Research, Christie Hospital NHS Trust, Manchester, UK. firstname.lastname@example.org
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AbstractScanning electron microscopy (SEM) has had a shorter time course in biology than conventional transmission electron microscopy (TEM) but has nevertheless produced a wealth of images that have significantly complemented our perception of biological structure and function from TEM information. By its nature, SEM is a surface imaging technology, and its impact at the subcellular level has been restricted by the considerably reduced resolution in conventional SEM in comparison to TEM. This restriction has been removed by the recent advent of high-brightness sources used in lensfield emission instruments (FEISEM) which have produced resolution of around 1 nanometre, which is not usually a limiting figure for biological material. This communication reviews our findings in the use of FEISEM in the imaging of nuclear surfaces, then associated structures, such as nuclear pore complexes, and the relationships of these structures with cytoplasmic and nucleoplasmic elements. High resolution SEM allows the structurally orientated cell biologist to visualise, directly and in three dimensions, subcellular structure and its modulation with a view to understanding, its functional significance. Clearly, intracellular surfaces require separation from surrounding structural elements in vivo to allow surface imaging, and we review a combination of biochemical and mechanical isolation methods for nuclear surfaces.
CitationAccessing nuclear structure for field emission, in lens, scanning electron microscopy (FEISEM). 1996, 10:149-63; discussion 163-4 Scanning Microsc. Suppl.
- Macromolecular substructure in nuclear pore complexes by in-lens field-emission scanning electron microscopy.
- Authors: Allen TD, Bennion GR, Rutherpord SA, Reipert S, Ramalho A, Kiseleva E, Goldberg MW
- Issue date: 1997 Sep
- Scanning electron microscopy of nuclear structure.
- Authors: Allen TD, Rutherford SA, Murray S, Drummond SP, Goldberg MW, Kiseleva E
- Issue date: 2008
- The use of field emission in-lens scanning electron microscopy to study the steps of assembly of the nuclear envelope in vitro.
- Authors: Goldberg MW, Blow JJ, Allen TD
- Issue date: 1992 May-Jun
- The nuclear pore complex: three-dimensional surface structure revealed by field emission, in-lens scanning electron microscopy, with underlying structure uncovered by proteolysis.
- Authors: Goldberg MW, Allen TD
- Issue date: 1993 Sep
- Visualization of the nucleus and nuclear envelope in situ by SEM in tissue culture cells.
- Authors: Allen TD, Rutherford SA, Murray S, Gardiner F, Kiseleva E, Goldberg MW, Drummond SP
- Issue date: 2007