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    Immunohistochemical examination of the expression of O6-methylguanine-DNA methyltransferase in human melanoma metastases.

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    Authors
    Egyházi, S
    Margison, Geoffrey P
    Hansson, J
    Ringborg, U
    Affiliation
    Department of Oncology, Karolinska Hospital, Stockholm, Sweden.
    Issue Date
    1997-01
    
    Metadata
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    Abstract
    In tumour cell lines, an inverse relationship has been shown between susceptibility to the cytotoxic effects of the O6-alkylating agents and the expression of the DNA repair protein O6-methylguanine-DNA methyltransferase (MGMT). One of the most effective single agents in chemotherapy of metastatic melanoma is the O6-alkylating drug, dacarbazine. We therefore examined the distribution of MGMT in 37 skin and lymph node melanoma metastases using rabbit antihuman MGMT antiserum. MGMT expression was undetectable in tumours from 2 out of 34 patients and low in 4 further patients. When present, staining was mainly nuclear and showed a marked variation both among tumour cells within the same metastases, between separate metastases in the same patient and between tumours in different patients. MGMT expression determined by immunohistochemistry showed a relation to MGMT activity measurements, but was not related to the number of proliferating cells, as identified by staining with MIB-1 antibody. Tumour cells with moderate to strong immunostaining with MGMT antiserum were significantly more abundant in metastases excised after dacarbazine-based chemotherapy (n = 8) than in those excised before treatment (n = 29).
    Citation
    Immunohistochemical examination of the expression of O6-methylguanine-DNA methyltransferase in human melanoma metastases. 1997, 33 (1):129-34 Eur. J. Cancer
    Journal
    European Journal of Cancer
    URI
    http://hdl.handle.net/10541/95257
    DOI
    10.1016/S0959-8049(96)00342-5
    PubMed ID
    9071912
    Type
    Article
    Language
    en
    ISSN
    0959-8049
    ae974a485f413a2113503eed53cd6c53
    10.1016/S0959-8049(96)00342-5
    Scopus Count
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