Macromolecular substructure in nuclear pore complexes by in-lens field-emission scanning electron microscopy.
AuthorsAllen, Terence D
Bennion, G R
Rutherford, S A
Goldberg, Martin W
AffiliationCRC Department of Structural Cell Biology, Paterson Institute for Cancer Research, Christie Hospital NHS Trust, Manchester, U.K.
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AbstractScanning electron microscopy (SEM) has produced a wealth of novel images that have significantly complemented our perception of biological structure and function, derived initially from transmission electron microscopy (TEM) information. SEM is a surface imaging technology, and its impact at the subcellular level has been restricted by reduced resolution in comparison with TEM. Recently, SEM resolution has been considerably improved by the advent of high-brightness sources used in field-emission instruments (FEISEM) which have produced resolution of around 1 nm, virtually equivalent to TEM "working resolution." Here we review our findings in the use of FEISEM in the imaging of nuclear envelopes and their associated structures, such as nuclear pore complexes, and the relationships of structure and function. FEISEM allows the structurally orientated cell biologist to visualise, directly and in three dimensions, subcellular structure and its modulation with a view to understanding its functional significance.
CitationMacromolecular substructure in nuclear pore complexes by in-lens field-emission scanning electron microscopy. 1997, 19 (6):403-10 Scanning
- Accessing nuclear structure for field emission, in lens, scanning electron microscopy (FEISEM).
- Authors: Allen TD, Bennion GR, Rutherford SA, Reipert S, Ramalho A, Kiseleva E, Goldberg MW
- Issue date: 1996
- The use of field emission in-lens scanning electron microscopy to study the steps of assembly of the nuclear envelope in vitro.
- Authors: Goldberg MW, Blow JJ, Allen TD
- Issue date: 1992 May-Jun
- Scanning electron microscopy of nuclear structure.
- Authors: Allen TD, Rutherford SA, Murray S, Drummond SP, Goldberg MW, Kiseleva E
- Issue date: 2008
- The nuclear pore complex: three-dimensional surface structure revealed by field emission, in-lens scanning electron microscopy, with underlying structure uncovered by proteolysis.
- Authors: Goldberg MW, Allen TD
- Issue date: 1993 Sep
- Visualization of the nucleus and nuclear envelope in situ by SEM in tissue culture cells.
- Authors: Allen TD, Rutherford SA, Murray S, Gardiner F, Kiseleva E, Goldberg MW, Drummond SP
- Issue date: 2007