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dc.contributor.authorBecciolini, A
dc.contributor.authorBalzi, M
dc.contributor.authorBarbarisi, M
dc.contributor.authorFaraoni, P
dc.contributor.authorBiggeri, A
dc.contributor.authorPotten, Christopher S
dc.date.accessioned2010-03-29T15:27:30Z
dc.date.available2010-03-29T15:27:30Z
dc.date.issued1997
dc.identifier.citation3H-thymidine labelling index (TLI) as a marker of tumour growth heterogeneity: evaluation in human solid carcinomas., 30 (3-4):117-26 Cell Prolif.en
dc.identifier.issn0960-7722
dc.identifier.pmid9375024
dc.identifier.doi10.1111/j.1365-2184.1997.tb00928.x
dc.identifier.urihttp://hdl.handle.net/10541/95186
dc.description.abstractMany studies deal with the analysis of cell kinetic, cytogenetic, biochemical and molecular cell biology parameters to identify prognostic factors relating to tumour growth but all methods use only a small part of the total tumour mass. This study is devoted to the analysis of the heterogeneity of the growth of human solid tumours assaying proliferative activity by means of 3H-thymidine labelling index (TLI) in a fixed number of samples collected in different areas of the lesion (larynx and colon cancers), or in different lesions of the same subject (breast and bladder cancers). Each sample (at the macroscopic level) was divided into small fragments (at the microscopic level) and proliferative activity was determined. The analysis of variance for hierarchical designs demonstrated that in all cases a high component of the variance is attributable to the subjects and to the fragments whereas the variance attributable to the different areas is very low. The heterogeneity of proliferative activity displays a higher focal variability among the fragments (microscopic level) compared with that among areas (macroscopic level) within subjects, provided an adequate number of fragments and cells are counted. In multiple synchronous carcinoma of the bladder the wide variability of proliferation among the single lesions demonstrated that it is necessary to analyse all the tumours in a subject because each one is characterized by a different cell growth potential.
dc.language.isoenen
dc.subjectBreast Canceren
dc.subjectColonic Canceren
dc.subjectLaryngeal Canceren
dc.subjectUrinary Bladder Canceren
dc.subject.meshAdult
dc.subject.meshAged
dc.subject.meshBreast Neoplasms
dc.subject.meshCarcinoma
dc.subject.meshCell Division
dc.subject.meshColonic Neoplasms
dc.subject.meshCulture Techniques
dc.subject.meshEvaluation Studies as Topic
dc.subject.meshFemale
dc.subject.meshHumans
dc.subject.meshLaryngeal Neoplasms
dc.subject.meshMiddle Aged
dc.subject.meshMitotic Index
dc.subject.meshThymidine
dc.subject.meshTritium
dc.subject.meshUrinary Bladder Neoplasms
dc.title3H-thymidine labelling index (TLI) as a marker of tumour growth heterogeneity: evaluation in human solid carcinomas.en
dc.typeArticleen
dc.contributor.departmentDepartment of Clinical Physiopathology, University of Florence, Italy.en
dc.identifier.journalCell Proliferationen
refterms.dateFOA2020-04-21T09:27:07Z
html.description.abstractMany studies deal with the analysis of cell kinetic, cytogenetic, biochemical and molecular cell biology parameters to identify prognostic factors relating to tumour growth but all methods use only a small part of the total tumour mass. This study is devoted to the analysis of the heterogeneity of the growth of human solid tumours assaying proliferative activity by means of 3H-thymidine labelling index (TLI) in a fixed number of samples collected in different areas of the lesion (larynx and colon cancers), or in different lesions of the same subject (breast and bladder cancers). Each sample (at the macroscopic level) was divided into small fragments (at the microscopic level) and proliferative activity was determined. The analysis of variance for hierarchical designs demonstrated that in all cases a high component of the variance is attributable to the subjects and to the fragments whereas the variance attributable to the different areas is very low. The heterogeneity of proliferative activity displays a higher focal variability among the fragments (microscopic level) compared with that among areas (macroscopic level) within subjects, provided an adequate number of fragments and cells are counted. In multiple synchronous carcinoma of the bladder the wide variability of proliferation among the single lesions demonstrated that it is necessary to analyse all the tumours in a subject because each one is characterized by a different cell growth potential.


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