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dc.contributor.authorBrooks, B
dc.contributor.authorBundred, Nigel J
dc.contributor.authorHowell, Anthony
dc.contributor.authorLang, Shona H
dc.contributor.authorTesta, Nydia G
dc.date.accessioned2010-03-24T14:34:03Z
dc.date.available2010-03-24T14:34:03Z
dc.date.issued1997-11-27
dc.identifier.citationInvestigation of mammary epithelial cell-bone marrow stroma interactions using primary human cell culture as a model of metastasis. 1997, 73 (5):690-6 Int. J. Canceren
dc.identifier.issn0020-7136
dc.identifier.pmid9398047
dc.identifier.doi10.1002/(SICI)1097-0215(19971127)73:5<690::AID-IJC13>3.0.CO;2-A
dc.identifier.urihttp://hdl.handle.net/10541/94889
dc.description.abstractA model has been established using primary human cell culture to study the cell biology of breast cancer metastasis to bone marrow. Mammary epithelia were obtained in single cell suspension from tumour (macroscopically involved), benign (macroscopically uninvolved) and normal (reduction mammoplasty) breast tissue as well as from locally involved lymph nodes. Stromal layers were generated from long-term cultures of human bone marrow or from mammary fibroblasts derived from normal or malignant tissue. The interaction between epithelia and stroma has been studied in terms of adhesion of the epithelia to the stroma and their subsequent growth in co-culture. Our results show that when assayed up to 9 hr after plating, epithelial cells from malignant tissue (14 primary tumours and 9 metastases in lymph nodes) displayed a significant preference for adhesion to bone marrow stroma compared with mammary fibroblasts. In contrast, epithelial cells from 4 normal and 2 of 4 benign samples showed no significant preferential adherence. Subsequent co-culture of mammary epithelia with each of the 3 stromal layers revealed that under serum-free, in vitro conditions, bone marrow stromal layers did not provide an advantageous environment for colony growth, in contrast to their ability to provide a preferential substratum for adhesion.
dc.language.isoenen
dc.subjectBreast Canceren
dc.subjectCancer Metastasisen
dc.subjectCultured Tumour Cellsen
dc.subject.meshAnimals
dc.subject.meshBone Marrow Cells
dc.subject.meshBreast
dc.subject.meshBreast Neoplasms
dc.subject.meshCell Adhesion
dc.subject.meshCell Communication
dc.subject.meshCell Division
dc.subject.meshCells, Cultured
dc.subject.meshCoculture Techniques
dc.subject.meshEpidermal Growth Factor
dc.subject.meshEpithelial Cells
dc.subject.meshFemale
dc.subject.meshFibroblasts
dc.subject.meshHumans
dc.subject.meshImmunohistochemistry
dc.subject.meshLymph Nodes
dc.subject.meshModels, Biological
dc.subject.meshNeoplasm Metastasis
dc.subject.meshStromal Cells
dc.subject.meshTumor Cells, Cultured
dc.titleInvestigation of mammary epithelial cell-bone marrow stroma interactions using primary human cell culture as a model of metastasis.en
dc.typeArticleen
dc.contributor.departmentCRC Department of Experimental Haematology, Paterson Institute for Cancer Research, Manchester, UK. ptbmb@liv.ac.uken
dc.identifier.journalInternational Journal of Cancer.en
html.description.abstractA model has been established using primary human cell culture to study the cell biology of breast cancer metastasis to bone marrow. Mammary epithelia were obtained in single cell suspension from tumour (macroscopically involved), benign (macroscopically uninvolved) and normal (reduction mammoplasty) breast tissue as well as from locally involved lymph nodes. Stromal layers were generated from long-term cultures of human bone marrow or from mammary fibroblasts derived from normal or malignant tissue. The interaction between epithelia and stroma has been studied in terms of adhesion of the epithelia to the stroma and their subsequent growth in co-culture. Our results show that when assayed up to 9 hr after plating, epithelial cells from malignant tissue (14 primary tumours and 9 metastases in lymph nodes) displayed a significant preference for adhesion to bone marrow stroma compared with mammary fibroblasts. In contrast, epithelial cells from 4 normal and 2 of 4 benign samples showed no significant preferential adherence. Subsequent co-culture of mammary epithelia with each of the 3 stromal layers revealed that under serum-free, in vitro conditions, bone marrow stromal layers did not provide an advantageous environment for colony growth, in contrast to their ability to provide a preferential substratum for adhesion.


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