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    Investigation of mammary epithelial cell-bone marrow stroma interactions using primary human cell culture as a model of metastasis.

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    Authors
    Brooks, B
    Bundred, Nigel J
    Howell, Anthony
    Lang, Shona H
    Testa, Nydia G
    Affiliation
    CRC Department of Experimental Haematology, Paterson Institute for Cancer Research, Manchester, UK. ptbmb@liv.ac.uk
    Issue Date
    1997-11-27
    
    Metadata
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    Abstract
    A model has been established using primary human cell culture to study the cell biology of breast cancer metastasis to bone marrow. Mammary epithelia were obtained in single cell suspension from tumour (macroscopically involved), benign (macroscopically uninvolved) and normal (reduction mammoplasty) breast tissue as well as from locally involved lymph nodes. Stromal layers were generated from long-term cultures of human bone marrow or from mammary fibroblasts derived from normal or malignant tissue. The interaction between epithelia and stroma has been studied in terms of adhesion of the epithelia to the stroma and their subsequent growth in co-culture. Our results show that when assayed up to 9 hr after plating, epithelial cells from malignant tissue (14 primary tumours and 9 metastases in lymph nodes) displayed a significant preference for adhesion to bone marrow stroma compared with mammary fibroblasts. In contrast, epithelial cells from 4 normal and 2 of 4 benign samples showed no significant preferential adherence. Subsequent co-culture of mammary epithelia with each of the 3 stromal layers revealed that under serum-free, in vitro conditions, bone marrow stromal layers did not provide an advantageous environment for colony growth, in contrast to their ability to provide a preferential substratum for adhesion.
    Citation
    Investigation of mammary epithelial cell-bone marrow stroma interactions using primary human cell culture as a model of metastasis. 1997, 73 (5):690-6 Int. J. Cancer
    Journal
    International Journal of Cancer.
    URI
    http://hdl.handle.net/10541/94889
    DOI
    10.1002/(SICI)1097-0215(19971127)73:5<690::AID-IJC13>3.0.CO;2-A
    PubMed ID
    9398047
    Type
    Article
    Language
    en
    ISSN
    0020-7136
    ae974a485f413a2113503eed53cd6c53
    10.1002/(SICI)1097-0215(19971127)73:5<690::AID-IJC13>3.0.CO;2-A
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    All Paterson Institute for Cancer Research

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