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dc.contributor.authorLi, C G
dc.contributor.authorKumar, S
dc.contributor.authorLedger, P W
dc.contributor.authorPonting, J M
dc.contributor.authorCarette, M
dc.contributor.authorAllan, Ernest
dc.date.accessioned2010-03-23T15:50:53Z
dc.date.available2010-03-23T15:50:53Z
dc.date.issued1997-09
dc.identifier.citationGlucosaminylmuramyl dipeptide (GMDP) modulates endothelial cell activities in vitro but has no effect on angiogenesis in vivo. 1997, 46 (9):348-53 Inflamm. Res.en
dc.identifier.issn1023-3830
dc.identifier.pmid9339390
dc.identifier.doi10.1007/s000110050200
dc.identifier.urihttp://hdl.handle.net/10541/94706
dc.description.abstractOBJECTIVE AND DESIGN: The aim of the study was to evaluate the effects of GMDP on angiogenesis in vivo and as a modulator of human umbilical vein endothelial cell proliferation, cell surface antigen expression and cell adhesion in vitro. MATERIALS: Human umbilical vein endothelial cells (HUVEC), fertilized white leghorn chicken eggs, antibodies against adhesion molecules and glucosaminylmuramyl dipeptide (GMDP). TREATMENT: GMDP [0.01-100 micrograms/ml] applied to cell cultures for 6-72 h and to the chick chorioallantoic membrane (CAM) for four days. METHODS: Angiogenic activity of GMDP in vivo was assessed using the CAM assay; HUVEC proliferation was measured by tritiated thymidine incorporation and cell cycle studies; cell surface antigen expression by indirect immunofluorescence and flow cytometry; cell adhesion by quantification of [3H]-thymidine labeled leukocyte adherence to HUVEC monolayers. Statistical analysis was performed using one-way ANOVA and if necessary was followed by Duncan's multiple range test for variables. RESULTS: GMDP induced [3H]-thymidine incorporation in a concentration- and time-dependent manner (p < 0.003) and significantly increased the porportion of cells in the S phase of the cell cycle (p < 0.03). It weakly augmented the expression of ICAM-1 and CD31 but not adhesion of leukocytes to HUVEC monolayers GMDP was not angiogenic in the CAM assay. CONCLUSIONS: GMDP can modulate endothelial cell activity without the induction of angiogenesis in vivo which may have implications for its use as a therapeutic agent.
dc.language.isoenen
dc.subject.meshAcetylmuramyl-Alanyl-Isoglutamine
dc.subject.meshAdjuvants, Immunologic
dc.subject.meshAnimals
dc.subject.meshAntigens, CD31
dc.subject.meshCell Adhesion
dc.subject.meshCell Cycle
dc.subject.meshCell Division
dc.subject.meshCell Line
dc.subject.meshCells, Cultured
dc.subject.meshChick Embryo
dc.subject.meshDNA
dc.subject.meshEndothelium, Vascular
dc.subject.meshHumans
dc.subject.meshIntercellular Adhesion Molecule-1
dc.subject.meshLeukocytes
dc.subject.meshNeovascularization, Physiologic
dc.subject.meshUmbilical Veins
dc.titleGlucosaminylmuramyl dipeptide (GMDP) modulates endothelial cell activities in vitro but has no effect on angiogenesis in vivo.en
dc.typeArticleen
dc.contributor.departmentDepartment of Pathology and Rheumatology, Medical School, University of Manchester, UK.en
dc.identifier.journalInflammation Researchen
html.description.abstractOBJECTIVE AND DESIGN: The aim of the study was to evaluate the effects of GMDP on angiogenesis in vivo and as a modulator of human umbilical vein endothelial cell proliferation, cell surface antigen expression and cell adhesion in vitro. MATERIALS: Human umbilical vein endothelial cells (HUVEC), fertilized white leghorn chicken eggs, antibodies against adhesion molecules and glucosaminylmuramyl dipeptide (GMDP). TREATMENT: GMDP [0.01-100 micrograms/ml] applied to cell cultures for 6-72 h and to the chick chorioallantoic membrane (CAM) for four days. METHODS: Angiogenic activity of GMDP in vivo was assessed using the CAM assay; HUVEC proliferation was measured by tritiated thymidine incorporation and cell cycle studies; cell surface antigen expression by indirect immunofluorescence and flow cytometry; cell adhesion by quantification of [3H]-thymidine labeled leukocyte adherence to HUVEC monolayers. Statistical analysis was performed using one-way ANOVA and if necessary was followed by Duncan's multiple range test for variables. RESULTS: GMDP induced [3H]-thymidine incorporation in a concentration- and time-dependent manner (p < 0.003) and significantly increased the porportion of cells in the S phase of the cell cycle (p < 0.03). It weakly augmented the expression of ICAM-1 and CD31 but not adhesion of leukocytes to HUVEC monolayers GMDP was not angiogenic in the CAM assay. CONCLUSIONS: GMDP can modulate endothelial cell activity without the induction of angiogenesis in vivo which may have implications for its use as a therapeutic agent.


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