Glucosaminylmuramyl dipeptide (GMDP) modulates endothelial cell activities in vitro but has no effect on angiogenesis in vivo.
AffiliationDepartment of Pathology and Rheumatology, Medical School, University of Manchester, UK.
MetadataShow full item record
AbstractOBJECTIVE AND DESIGN: The aim of the study was to evaluate the effects of GMDP on angiogenesis in vivo and as a modulator of human umbilical vein endothelial cell proliferation, cell surface antigen expression and cell adhesion in vitro. MATERIALS: Human umbilical vein endothelial cells (HUVEC), fertilized white leghorn chicken eggs, antibodies against adhesion molecules and glucosaminylmuramyl dipeptide (GMDP). TREATMENT: GMDP [0.01-100 micrograms/ml] applied to cell cultures for 6-72 h and to the chick chorioallantoic membrane (CAM) for four days. METHODS: Angiogenic activity of GMDP in vivo was assessed using the CAM assay; HUVEC proliferation was measured by tritiated thymidine incorporation and cell cycle studies; cell surface antigen expression by indirect immunofluorescence and flow cytometry; cell adhesion by quantification of [3H]-thymidine labeled leukocyte adherence to HUVEC monolayers. Statistical analysis was performed using one-way ANOVA and if necessary was followed by Duncan's multiple range test for variables. RESULTS: GMDP induced [3H]-thymidine incorporation in a concentration- and time-dependent manner (p < 0.003) and significantly increased the porportion of cells in the S phase of the cell cycle (p < 0.03). It weakly augmented the expression of ICAM-1 and CD31 but not adhesion of leukocytes to HUVEC monolayers GMDP was not angiogenic in the CAM assay. CONCLUSIONS: GMDP can modulate endothelial cell activity without the induction of angiogenesis in vivo which may have implications for its use as a therapeutic agent.
CitationGlucosaminylmuramyl dipeptide (GMDP) modulates endothelial cell activities in vitro but has no effect on angiogenesis in vivo. 1997, 46 (9):348-53 Inflamm. Res.
- Fluid shear stress modulates surface expression of adhesion molecules by endothelial cells.
- Authors: Morigi M, Zoja C, Figliuzzi M, Foppolo M, Micheletti G, Bontempelli M, Saronni M, Remuzzi G, Remuzzi A
- Issue date: 1995 Apr 1
- Antiangiogenic potential of camptothecin and topotecan.
- Authors: Clements MK, Jones CB, Cumming M, Daoud SS
- Issue date: 1999
- Effect of U-995, a potent shark cartilage-derived angiogenesis inhibitor, on anti-angiogenesis and anti-tumor activities.
- Authors: Sheu JR, Fu CC, Tsai ML, Chung WJ
- Issue date: 1998 Nov-Dec
- Endogenous tumour necrosis factor-alpha sensitise melanoma cells to glucosaminylmuramyl dipeptide.
- Authors: Valyakina TI, Komaleva RL, Petrova EE, Malakhov AA, Shamborant OG, Andronova TM, Nesmeyanov VA
- Issue date: 1998 Apr 24
- [Effect of mycophenolic acid and dexamethasone on the angiogenic activity of human umbilical vein endothelial cells].
- Authors: Huang H, Liu Z, Liu H
- Issue date: 2001 Jul 10